wnvNS1 Protein Antibody (991501) [Unconjugated]

Western blot shows recombinant West Nile Virus (wnv) NS1 protein. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral wnvNS1 Protein Monoclonal Antibody (Catalog # MAB29072) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for wnvNS1 Protein at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB
• Clone: 991501
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

wnvNS1 Protein Antibody (991501) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with recombinant Viral wnvNS1 Protein
  Asp1-Ala352
  Accession # NP_776015
• Specificity: Detects viral wnvNS1 Protein in direct ELISAs. In direct ELISAs, no cross-reactivity with viral Dengue NS1 (serotypes 1,2 and 3) and viral Zika NS1 proteins is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for wnvNS1 Protein Antibody (991501) [Unconjugated]

• wnvNS1 Protein

Background

West Nile Virus, or WNV, is a member of the Flaviviridae family that includes other human pathogens such as yellow fever (YF), dengue (DEN), tick-borne encephalitis (TBE), Japanese encephalitis (JEV), and Murray Valley encephalitis (MVE) viruses. West Nile virus (WNV) is a single-stranded RNA mosquito-transmitted virus that causes epidemics of encephalitis and viscerotropic disease. The WNV nonstructural protein NS1 is a 48-kDa highly conserved secreted glycoprotein that does not package with the virion. Even when absent from the virion, immunization with NS1 typically elicits a protective immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • NP_776015()