VSIG10 Antibody (988622) [Unconjugated]

VSIG10 was detected in immersion fixed RT-4 human urinary bladder transitional cell papilloma cell line (left panel) and MOLT-4 human acute lymphoblastic leukemia cell line (negative control; right panel) using Mouse Anti-Human VSIG10 Monoclonal Antibody (Catalog # MAB9969) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 988622
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

VSIG10 Antibody (988622) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived human VSIG10
  Met1-Gly413
  Accession # Q8N0Z9
• Specificity: Detects human VSIG10 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VSIG10 Antibody (988622) [Unconjugated]

• FLJ20674
• V-set and immunoglobulin domain containing 10
• V-set and immunoglobulin domain-containing protein 10
• VSIG10

Background

VSIG10 (V-set and immunoglobulin domain-containing protein 10) is a single-pass type I membrane protein of the Ig superfamily. Human VSIG10 cDNA encodes a 540 amino acid (aa) precursor that contains a 30 aa signal sequence, a 383 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 106 aa cytoplasmic domain. Human VSIG10 ECD contains four Ig-like C2-type domains and shares 95%, 69% and 71% aa identity with chimpanzee, mouse and rat VSIG10, respectively. VSIG10 is structurally related to the B7 family of immune regulatory proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8N0Z9()