VPS50 Antibody (2D11) - Azide and BSA Free

Western Blot: FLJ20097 Antibody (2D11) [H00055610-M01] - FLJ20097 monoclonal antibody (M01), clone 2D11. Analysis of FLJ20097 expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: FLJ20097 Antibody (2D11) [H00055610-M01] - Analysis of monoclonal antibody to FLJ20097 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: FLJ20097 Antibody (2D11) [H00055610-M01] - Analysis of monoclonal antibody to FLJ20097 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Western Blot: FLJ20097 Antibody (2D11) [H00055610-M01] - FLJ20097 monoclonal antibody (M01), clone 2D11 Analysis of FLJ20097 expression in HeLa.

Western Blot: FLJ20097 Antibody (2D11) [H00055610-M01] - FLJ20097 monoclonal antibody (M01), clone 2D11. Analysis of FLJ20097 expression in human pancreas.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

VPS50 Antibody (2D11) - Azide and BSA Free Summary

• Immunogen: FLJ20097 (NP_060137, 862 a.a. ~ 964 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GYANVKKCSNEGRALMQLDFQQFLMKLEKLTDIRPIPDKEFVETYIKAYYLTENDMERWIKEHREYSTKQLTNLVNVCLGSHINKKARQKLLAAIDDIDRPKR
• Specificity: FLJ20097 - hypothetical protein LOC55610, isoform b
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: VPS50
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for ELISA, immunofluorescence and immunohistochemistry (paraffin).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for VPS50 Antibody (2D11) - Azide and BSA Free

• CCDC132
• coiled-coil domain containing 132
• coiled-coil domain-containing protein 132
• EARP/GARPII Complex Subunit VPS50
• KIAA1861
• Syndetin
• VPS50, EARP/GARPII Complex Subunit
• VPS54L

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FLJ20097 Antibody (H00055610-M01)(5)

Publications using H00055610-M01

• Ishida M, Bonifacino J ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network. J Cell Biol. 2019-10-01 [PMID: 31575603]
• Shi Z, Chen S, Han X et al. The rare mutation in the endosome-associated recycling protein gene VPS50 is associated with human neural tube defects. Mol Cytogenet. 2019-02-20 [PMID: 30828385]
• Gershlick DC, Schindler C, Chen Y, Bonifacino JS. TSSC1 is novel component of the endosomal retrieval machinery. Mol Biol Cell 2016-09-15 [PMID: 27440922]
• Topalidou I, Cattin-Ortola J, Pappas AL et al. The EARP Complex and Its Interactor EIPR-1 Are Required for Cargo Sorting to Dense-Core Vesicles. PLoS Genet 2016-05-01 [PMID: 27191843] (WB)
• Schindler C, Chen Y, Pu J et al. EARP is a multisubunit tethering complex involved in endocytic recycling. Nat Cell Biol 2015-05-01 [PMID: 25799061]

Bioinformatics

• Gene Symbol: VPS50
• Entrez:
  • 55610(Human)
• Uniprot:
  • NP_060137(Human)