VAT1L Antibody (OTI1H3) - Azide and BSA Free

Western Blot: VAT1L Antibody (OTI1H3) - Azide and BSA Free [NBP2-74831] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAT1L (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VAT1L.

Immunocytochemistry/Immunofluorescence: VAT1L Antibody (OTI1H3) - Azide and BSA Free [NBP2-74831] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VAT1L.

Immunohistochemistry: VAT1L Antibody (OTI1H3) - Azide and BSA Free [NBP2-74831] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-VAT1L mouse monoclonal antibody.

Flow Cytometry: VAT1L Antibody (OTI1H3) - Azide and BSA Free [NBP2-74831] - HEK293T cells transfected with either pCMV6-ENTRY VAT1L.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-VAT1L mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry: VAT1L Antibody (OTI1H3) - Azide and BSA Free [NBP2-74831] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-VAT1L mouse monoclonal antibody.

Product Discontinued

VAT1L Antibody (OTI1H3) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human VAT1L (NP_065978) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAT1L
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 45.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for VAT1L Antibody (OTI1H3) - Azide and BSA Free

• KIAA1576synaptic vesicle membrane protein VAT-1 homolog-like
• vesicle amine transport protein 1 homolog (T. californica)-like
• vesicle amine transport protein 1 homolog-like (T. californica)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAT1L