VasoTACS In Situ Apoptosis Kit [HRP]

Immunohistochemistry: VasoTACS In Situ Apoptosis Kit [HRP] [NB4826-30-K] - VasoTACS In Situ Apoptosis Kit [TACS Blue Label] [NB4826-30-K] - Visualization of Apoptotic Cells in Vascular Tissue: TIssue from the small artery of rat was formalin-fixed and paraffin-embedded following the initiation of drug-induced apoptosis. The tissue was processed according to the protocol in the VasoTACS Kit (NB4826-30-K) and visualized by light microscopy. Brown stained nuclei following counterstaining with Red Counterstain C indicated apoptotic cells. Data was provided courtesy of Dr. Jun Zhang, FDA.

Product Discontinued

VasoTACS In Situ Apoptosis Kit [HRP] Summary

• Description: TUNEL Apoptosis Detection Kit provides all the reagents for the detection of DNA fragmentation among vascular cells and tissue sections. It generates an intense blue staining in cells with DNA fragmentation, easily visualized against a pale red counterstain. In addition, Cytonin, a non-lipophilic detergent based buffer optimized for the permeabilization of cells prior to labeling, and Blue Streptavidin Diluent, a proprietary blocking reagent that contributes to the low background obtained with Tunel Apoptosis Detection kit are provided.
  
  Detection: Biotinylated dNTP/Streptavidin-HRP/Blue Label
  Testing Format: Light Microscopy
  Sample Type: Fixed cells, Embedded tissues, Frozen tissues
• Kit Type: Apoptosis Kit

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Background

Apoptosis is characterized by a number of intracellular phenomena such as membrane blebbing, chromatin condensation and nuclear DNA fragmentation. Detection of nuclear DNA fragmentation is a widely accepted method to assay for apoptosis and can be performed in situ by incorporating labeled nucleotides onto the free 3' OH ends of DNA fragments using a terminal deoxynucleotidyl transferase enzyme (TdT). Terminal dUTP nick-end labeling (TUNEL) allows monitoring of apoptotic events among cells and within tissue sections. The incorporation of biotinylated nucleotides allows chromosomal DNA fragmentation to be visualized by binding streptavidin-horseradish peroxidase followed by reaction with TACS Blue Label(TM) to generate a dark blue precipitate. When viewed under a standard light microscope apoptotic cells are clearly distinguished by dark blue staining.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.