Description
Apoptosis is characterized by a number of intracellular phenomena such as membrane blebbing, chromatin condensation and nuclear DNA fragmentation. Detection of nuclear DNA fragmentation is a widely accepted method to assay for apoptosis and can be performed in situ by incorporating labeled nucleotides onto the free 3' OH ends of DNA fragments using a terminal deoxynucleotidyl transferase enzyme (TdT). Terminal dUTP nick-end labeling (TUNEL) allows monitoring of apoptotic events among cells and within tissue sections. The incorporation of biotinylated nucleotides allows chromosomal DNA fragmentation to be visualized by binding streptavidin-horseradish peroxidase followed by reaction with TACS Blue Label(TM) to generate a dark blue precipitate. When viewed under a standard light microscope apoptotic cells are clearly distinguished by dark blue staining.
Research Areas for VasoTACS In Situ
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ApoptosisApoptosis Detections
