![Western Blot: V5 Epitope Tag Antibody - Bulk Size [NB600-384] - NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). NB600-381 was used at 0.2 mcg/ml (1:5000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Bulk-Size-Western-Blot-NB600-384-img0005.jpg "Western Blot: V5 Epitope Tag Antibody - Bulk Size [NB600-384] - NP-40 whole cell lysate (10 ug) from wild-type HEK293 cells (lane 1) or HEK293 cells expressing V5 tagged Nicastrin (lane 2). NB600-381 was used at 0.2 mcg/ml (1:5000).")
| Immunogen | Rabbits were immunized with a synthetic peptide representing amino acid residues 95 to 108 of RNA polymerase alpha subunit of simion virus 5 conjugated to KLH. |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for V5 Epitope Tag Antibody (NB600-384)Find related products by research area.
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V5 Tags are preferable for detection of Exogenous Proteins in Vivo Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of... Read full blog post. |
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Working with the V5 Tag Antibody The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit... Read full blog post. |
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![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody - Bulk Size [NB600-384] - Drosophila adult midgut expressing a V5-tagged protein (magenta) using the GAL4/UAS system. Nuclei are shown in green. pAb dilution of 1:500. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody---Bulk-Size-Immunocytochemistry-Immunofluorescence-NB600-384-img0004.jpg "Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody - Bulk Size [NB600-384] - Drosophila adult midgut expressing a V5-tagged protein (magenta) using the GAL4/UAS system. Nuclei are shown in green. pAb dilution of 1:500. This image was submitted via customer Review.")
![Western Blot: V5 Epitope Tag Antibody - Bulk Size [NB600-384] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody---Bulk-Size-Western-Blot-NB600-384-img0002.jpg "Western Blot: V5 Epitope Tag Antibody - Bulk Size [NB600-384] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
![Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody - Bulk Size [NB600-384] - HEK293 cells transiently expressing V5-tagged mutant PFN1 stained with V5 Epitope Tag antibody (red). Image from verified customer review.](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody---Bulk-Size-Immunocytochemistry-Immunofluorescence-NB600-384-img0003.jpg "Immunocytochemistry/Immunofluorescence: V5 Epitope Tag Antibody - Bulk Size [NB600-384] - HEK293 cells transiently expressing V5-tagged mutant PFN1 stained with V5 Epitope Tag antibody (red). Image from verified customer review.")