V5 Epitope Tag Antibody

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Western Blot: V5 Epitope Tag Antibody [NB600-383] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

V5 Epitope Tag Antibody Summary

Immunogen
Goats were immunized with V5 cleavage site (GKPIPNPLLGLDST) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Goat anti-human V5 affinity purified antibodies were coupled to peptide immobilized on solid support.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:20000
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/Immunofluorescence 1:100-1:400
  • Immunoprecipitation 1 - 4 ug/mg lysate
Application Notes
Suggested working dilutions: * ELISA Coating 1:100-1:500 Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 cyto 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot and immunocytochemistry.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for V5 Epitope Tag Antibody

  • GKPIPNPLLGLDST Epitope Tag
  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 Epitope Tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for V5 Epitope Tag Antibody (NB600-383) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Agarose Immobilized Labeled NB600-387
Biotin Labeled NB600-380B
FITC Labeled NB600-385
HRP Labeled NB600-386

Additional V5 Epitope Tag Products

V5 Epitope Tag NB600-383

Research Areas for V5 Epitope Tag Antibody (NB600-383)

Find related products by research area.

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

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