![Western Blot: V5 Epitope Tag Antibody (SV5-Pk2) [NBP1-21645] - V5 tagged protein detected with Mouse anti V5-tag](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-SV5-Pk2-Western-Blot-NBP1-21645-img0001.jpg "Western Blot: V5 Epitope Tag Antibody (SV5-Pk2) [NBP1-21645] - V5 tagged protein detected with Mouse anti V5-tag")
| Immunogen | A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope. |
| Specificity | NBP1-21645 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. NBP1-21645 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined). |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for V5 Epitope Tag Antibody (NBP1-21645)Find related products by research area.
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V5 Tags are preferable for detection of Exogenous Proteins in Vivo Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of... Read full blog post. |
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Working with the V5 Tag Antibody The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit... Read full blog post. |
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