V5 Epitope Tag Antibody (E10/V4RR) [Janelia Fluor® 585]

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-37825JF585
    • Availability
      Product Discontinued

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V5 Epitope Tag Antibody (E10/V4RR) [Janelia Fluor® 585] Summary

Immunogen
GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Specificity
This product recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins. It has successfully been used in immunoprecipitation, ELISA, Western blotting, and immunofluorescence applications.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for V5 Epitope Tag Antibody (E10/V4RR) [Janelia Fluor® 585]

  • GKPIPNPLLGLDST Epitope Tag
  • Parainfluenza virus 5 V/P
  • Paramyxovirus SV5
  • V5 Epitope Tag

Background

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional V5 Epitope Tag Products

Array NBP2-37825JF585

Blogs on V5 Epitope Tag.

V5 Tags are preferable for detection of Exogenous Proteins in Vivo
Expression of transgenes in complex organisms is accompanied by the need for a specific and sensitive detection of a target protein. One familiar approach is the use of a protein-specific antibody. However, antibodies raised against to your protein of...  Read full blog post.

Working with the V5 Tag Antibody
The V5 tag antibody identifies the V5 epitope tag, enabling proteins of interest to be analyzed and studied via ELISA, Western blot and other immunochemical methods. We at Novus Biologicals are one of the leading suppliers of epitope tag reagents, wit...  Read full blog post.

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