Ub-K63 Antibody (HWA4C4) - BSA Free

Western Blot: Ub-K63 Antibody (HWA4C4) [NBP2-00199] - In vitro linked and purified polyubiquitin chains were analyzed by SDS-PAGE followed by immunoblotting with Anti-Human Ub-K63 Purified. The antibody detects lysine-63-linked chains (lane 1), but does not detect lysine-48-linked chains (lane 2).

• Reactivity: Hu
• Applications: WB
• Clone: HWA4C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Ub-K63 Antibody (HWA4C4) - BSA Free Summary

• Immunogen: The immunogen for this antibody is Ub-K63.
• Specificity: HWA4C4 reacts specifically with the isopeptide linkage region of lysine-63-linked polyubiquitin. It does not react with other forms of ubiquitin including ubiquitin monomer and lysine-48-linked chains.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot < = 2 ug/ml
• Application Notes: This HWA4C4 antibody has been tested by immunoblot of lysine-63-linked polyubiquitin chains. This can be used at less than or equal to 2ug/ml . It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Background

Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63). K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ub-K63 Antibody (NBP2-00199)(2)

Publications using NBP2-00199

• Zhang YY, Tabataba H, Liu XY et al. ACTN4 regulates the stability of RIPK1 in melanoma Oncogene 2018-04-30 [PMID: 29706658] (WB, Human)
• Liu XY, Lai F, Yan XG et al. RIP1 Kinase Is an Oncogenic Driver in Melanoma Cancer Res. 2015-02-27 [PMID: 25724678] (WB, Human)

FAQs for Ub-K63 Antibody (NBP2-00199). (Showing 1 - 1 of 1 FAQs).

1. What is the buffer composition of this product?
   • The buffer formulation of NBP2-00199 (Anti-Human Ub-K63) is 1X PBS with 0.09% sodium azide, pH 7.2, and no carrier protein.