U-138 MG Whole Cell Lysate


SDS-Page: U-138 MG Whole Cell Lysate [NBP3-06999] - SDS-PAGE Results of U-138 MG Whole Cell Lysate. Lane 1: U-138 MG Whole Cell lysate(10ug) Reduced. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: U-138 MG ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, PAGE

Order Details

U-138 MG Whole Cell Lysate Summary

The U-138 MG cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 uM Aprotinin, 5 uM Bestatin, 1.5 uM E-64, 2 uM Leupeptin Hemisulfate, 1 uM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified SDS assay using a commercially available kit. Protein concentration was adjusted to 2 mg/mL and then an equal volume of 2X SDS-PAGE sample buffer was added.


  • SDS-Page
  • Western Blot
Application Notes
This product has been tested by SDS-PAGE. Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 uL depending on the size format of your gel.

Packaging, Storage & Formulations

Store at -70C. Avoid freeze-thaw cycles.
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
No Preservative

Lysate Details for Array

Subcellular Fraction


Store vial at -70C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for U-138 MG Lysate (NBP3-06999) (0)

There are no publications for U-138 MG Lysate (NBP3-06999).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for U-138 MG Lysate (NBP3-06999) (0)

There are no reviews for U-138 MG Lysate (NBP3-06999). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for U-138 MG Lysate (NBP3-06999) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional U-138 MG Products

Array NBP3-06999

Blogs on U-138 MG

There are no specific blogs for U-138 MG, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our U-138 MG Whole Cell Lysate and receive a gift card or discount.