Transcriptional regulator UME6 Antibody

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-UME6 )

Product Discontinued

Transcriptional regulator UME6 Antibody Summary

• Immunogen: The immunogen for this antibody was a sequence from 339a.a. to 808a.a. from Transcriptional regulator UME6(a 836a.a. long protein)
• Localization: Membrane; single-pass type I membrane protein.
• Specificity: Transcriptional regulator UME6
• Clonality: Polyclonal
• Host: Chicken
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

Reactivity Notes

This antibody is reactive against Yeast.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for Transcriptional regulator UME6 Antibody

• CAR80
• CARGR1
• Negative transcriptional regulator of IME2
• NIM2
• Regulator of inducer of meiosis protein 16
• RIM16
• Unscheduled meiotic gene expression protein 6

Background

UME6 is a component of the RPD3C(L) histone deacetylase complex (HDAC) responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. UME6 binds to the URS1 site (5'-AGCCGCCGA-3') and recruits the RPD3 histone deacetylase complex to the promoters to negatively regulate the expression of many genes including CAR1 (arginase), several required for sporulation, mating type switching, inositol metabolism, and oxidative carbon metabolism. Recruits also the ISW2 chromatin remodeling complex to promoters in a second gene repression pathway. UME6 also associates with the master regulator of meiosis IME1 in order to activate the expression of meiosis genes. It has both a positive and negative role in regulating phospholipid biosynthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.