Toxoplasma Gondii Antibody (TP3cc) - BSA Free

• Reactivity: Pr, Pz
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: TP3cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Toxoplasma Gondii Antibody (TP3cc) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Tachyzoites lysate.
• Epitope: P30 antigen
• Specificity: This Toxoplasma Gondii Antibody (TP3cc) reacts with p30 membrane protein in ELISA and immunoblotting
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Use in Flow reported in scientific literature (PMID: 32413093).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Toxoplasma Gondii Antibody (TP3cc) - BSA Free

• t gondii
• T. gondii

Background

Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Toxoplasma Gondii Antibody (NB110-2570)(1)

Publication using NB110-2570

• Mercer HL, Snyder LM, Doherty CM et al. Toxoplasma gondii dense granule protein GRA24 drives MyD88-independent p38 MAPK activation, IL-12 production and induction of protective immunity PLoS Pathog. 2020-05-01 [PMID: 32413093] (FLOW, Mouse)