TLR11 Antibody

Western Blot: TLR11 Antibody [NB100-56742] - WB analysis of TLR11 in mouse testis lysate in A) the absence and B) presence of blocking peptide and rat testis lysate in C) the absence and D) presence of blocking peptide using at 3 ug/ml.

Flow Cytometry: TLR11 Antibody [NB100-56742] - Analysis using the FITC conjugate of NB100-56742. Staining of TLR11 in the TLR11 stable cell line using this antibody at 1 ug/10^6 cells (green represents vector control, red represents TLR11 antibody).

Western Blot: TLR11 Antibody [NB100-56742] - Western blot analysis of TLR11 in mouse RAW cell lysate using TLR11 antibody at 2 ug/ml.

Flow Cytometry: TLR11 Antibody [NB100-56742] - Intracellular flow analysis of TLR11 in Balb/c mouse splenocyte at 0.5 ug/10^6 cells. Shaded histogram shows cells without ab; green shows rabbit IgG isotype control; red shows anti-TLR11 ab. Goat anti-rabbit PE was used as secondary ab.

Flow Cytometry: TLR11 Antibody [NB100-56742] - Analysis using the FITC conjugate of NB100-56742. Staining of TLR11 in Balb/c mouse splenocytes using TLR11 antibody at 1 ug/10^6 cells. (Green represents rabbit IgG isotype control. Red represents TLR11 antibody).

• Reactivity: Mu, Rt
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TLR11 Antibody Summary

• Immunogen: A synthetic peptide within amino acid residues 911-926 (CEQLKRRLSKAGQERD) of mouse TLR11 (Genbank accession no. NP_991388) was used as immunogen.
• Specificity: Immunizing peptide will block the mouse TLR11 band in western blotting. The approx. 32 kDa band may be a proteolytic degradation product of TLR11.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TLR11
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1 ug/10-6 cells
  • Western Blot 1-3 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TLR11 Antibody

• Gm287
• hgnc31753
• TLR11
• Toll like receptor 11

Background

Toll like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR11 is one of three mouse TLRs that lack a human ortholog. It is activated specifically by uropathogenic bacteria, and mice lacking TLR11 showed a much greater susceptability to uropathogenic infections, indicating a potentially important role for TLR11 in preventing infections in the urogenital system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR11
• Entrez:
  • 239081(Mouse)
  • 498491(Rat)
• Uniprot:
  • NP_991388()