Thyroxine Antibody (T4YCH)

Western Blot: Thyroxine Antibody (T4YCH) [NBP1-78604] - Analysis of L-Thyroxine was performed by loading 600 ng, 300 ng, 150 ng and 75 ng of a Thyroxine-BSA conjugate and 10 uL PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel.

ELISA: Thyroxine Antibody (T4YCH) [NBP1-78604] - Direct ELISA analysis of L-Thyroxine was performed by coating wells of a 96-well plate with 100 uL per well of L-Thyroxine-bsa conjugated protein diluted in carbonate/bicarbonate buffer.

• Reactivity: All-NA
• Applications: WB, ELISA, Func, IP
• Clone: T4YCH
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Thyroxine Antibody (T4YCH) Summary

• Description: Novus Biologicals Mouse Thyroxine Antibody (T4YCH) (NBP1-78604) is a monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Thyroxine-BSA
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunoprecipitation 1:10 - 1:500
  • Radioimmunoassay 1:100 - 1:2000
  • Western Blot

Reactivity Notes

Chemical

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA and 30% glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Thyroxine Antibody (T4YCH)

• T4

Background

Hormone produced by the thyroid glands to regulate metabolism by controlling the rate of oxidation in cells. Cases of hypothyroidism, where the gland is insufficiently active, can be treated by administration of thyroxine or a combination of thyroxine and triiodothyronine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.