Thymine Dimer Antibody (H3) - BSA Free

Immunocytochemistry/Immunofluorescence: Thymine Dimer Antibody (H3) [NB600-1141] - analysis of Thymine Dimer in human keratinocytes using anti-Thymine Dimer antibody. Image from verified customer review.

• Reactivity: Hu, All-NA, Ch
• Applications: DB, ELISA, ICC/IF
• Clone: H3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.0 mg/ml

Thymine Dimer Antibody (H3) - BSA Free Summary

• Immunogen: tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.
• Specificity: Monoclonal Anti-Thymine Dimer reacts specifically with the (5'-6') cyclobutane type of homothymine or thyminecytosine heterodimers. The antibody reacts with thymine dimers in single-stranded DNA, and has a lower affinity for the dimer in short oligonucleotides (a tail of minimum 10-20 thymine residues is required for efficient labeling of oligonucleotide probes). The product enables a sensitive and non-radioactive method for labeling, detection, and quantification of DNA fragments using ELISA, competitive ELISA, immunocytochemistry (laser-scan microscopy) and Southern immunoblotting.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot 0.5-1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
• Application Notes: Southern Blotting, use UV induced thymine dimers of lamda phage DNA fragments

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Background

Non-radioactive labeling of DNA is typically based on the enzymatic incorporation of modified nucleotides, carrying a small chemical moiety such as biotin, digoxigenin or fluorescein. These tags are subsequently detected by specific reagents such as streptavidin or a specific antibody coupled to a signal-producing enzyme. Although very efficient and reliable, labeling by in vitro polymerization is time-consuming, expensive, and may require various post-label purification steps to remove an excess of unincorporated precursors. An alternative strategy for DNA labeling, is based on the UV-induced formation of cyclobutane thymine dimers. Several methods have been described for the detection of thymine dimers, which are based on chromatographic analysis, and on biochemical analysis with endonucleases specific for UV-irradiated DNA. In addition, methods utilizing antibodies specific for pyrimidine dimers and other UV-induced DNA lesions have evolved, which permit the study of the induction and repair of these lesions without the requirement of in vivo radiolabeling of DNA. Photoimmunodetection is a rapid, reliable and low-cost supplement to existing methods for nonradioactive DNA labeling. It enables a sensitive and non-radioactive method for labeling, detection, and quantification of high molecular weight (HMW) DNA fragments. The method is based on the introduction of thymine dimers into DNA after separation by pulse field gel electrophoresis (PFGE), followed by detection with thymine dimer specific antibodies. This method does not require any enzymatic or chemical manipulation of the DNA sample. Monoclonal antibodies reacting specifically with thymine dimer facilitate investigations on the apoptotic process and the role of UV-induced pyrimidine dimers in the process of photocarcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thymine Dimer Antibody (NB600-1141)(4)

Publications using NB600-1141

• Wong W, Kim A, Monaghan JR et al. Spiny mice (Acomys) exhibit attenuated hallmarks of aging and rapid cell turnover after UV exposure in the skin epidermis PLOS ONE 2020-10-30 [PMID: 33125436] (Immunocytochemistry/ Immunofluorescence)
• Patil G Role of Pkc Delta in Uv Radiation Dna Damage Repair Thesis 2016-01-01 (ICC/IF, Human)
• Ong Q, Wee W, Dela Cruz J et al. 222-Nanometer Far-UVC Exposure Results in DNA Damage and Transcriptional Changes to Mammalian Cells International journal of molecular sciences 2022-08-14 [PMID: 36012379] (ICC/IF, Human)
• Wong W, Crane ED, Zhang H et al. Pgc-1 alpha controls epidermal stem cell fate and skin repair by sustaining NAD+ homeostasis during aging Molecular metabolism 2022-08-17 [PMID: 35987498] (IHC-P, Mouse)
  
  Details:
  Staining done on mouse skin samples, Dilution used 1:400

Review for Thymine Dimer Antibody (NB600-1141) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 05/12/2015

Summary

• Application: Immunofluorescence
• Sample Tested: Cultured Human Keratinocytes
• Species: Human
• Lot: 043M4836