TGF-beta 1/1.2 Antibody [Unconjugated]


Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of more

Product Details

Reactivity All-MultiSpecies Glossary
Applications WB, B/N

Order Details

View Available Formulations
Catalog# & Formulation Size Price

TGF-beta 1/1.2 Antibody [Unconjugated] Summary

Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1
Accession # P01137
Detects TGF-beta 1/1.2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with TGF-beta 2 is observed.
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.1 ug/mL
  • Neutralization 5-30 ng/mL
Read Publications using
AF-101-NA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 1/1.2 Antibody [Unconjugated]

  • TGFb1.2
  • TGF-beta 1/1.2


TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 1/1.2 Antibody (AF-101-NA)(25)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Guinea Pig, Mink, Rabbit.

We have publications tested in 8 applications: Blocking, Flow Cytometry, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.

Filter By Application
Flow Cytometry
Western Blot
All Applications
Filter By Species
Guinea Pig
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using AF-101-NA Applications Species
S Holvoet, M Perrot, N de Groot, G Prioult, T Mikogami, V Verhasselt, S Nutten Oral Tolerance Induction to Newly Introduced Allergen is Favored by a Transforming Growth Factor-beta-Enriched Formula Nutrients, 2019;11(9):. 2019 [PMID: 31540231] (Neutralization, Mink) Neutralization Mink
M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Int J Mol Sci, 2019;20(1):. 2019 [PMID: 30609691] (Neutralization, Human) Neutralization Human
O Miroshnych, WT Chang, JL Dragoo The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2017;45(4):945-953. 2017 [PMID: 28027451] (Immunoprecipitation, Human) Immunoprecipitation Human
Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment. Sci Rep, 2015;5(0):8919. 2015 [PMID: 25747469] (IHC, Mouse) IHC Mouse
Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta. Proc Natl Acad Sci U S A, 2014;111(21):E2229-36. 2014 [PMID: 24825889] (Immunoprecipitation, Human) Immunoprecipitation Human
Yeste A, Mascanfroni I, Nadeau M, Burns E, Tukpah A, Santiago A, Wu C, Patel B, Kumar D, Quintana F IL-21 induces IL-22 production in CD4+ T cells. Nat Commun, 2014;5(0):3753. 2014 [PMID: 24796415] (Neutralization, Mouse) Neutralization Mouse
Kang I, Yoon D, Braun K, Wight T Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion. J Biol Chem, 2014;289(22):15393-404. 2014 [PMID: 24719328] (Neutralization, Rat) Neutralization Rat
Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C Long-term gene therapy with thrombospondin 2 inhibits TGF-beta activation, inflammation and angiogenesis in chronic allograft nephropathy. PLoS ONE, 2013;8(12):e83846. 2013 [PMID: 24376766] (IHC, Rat) IHC Rat
Jelicic K, Cimbro R, Nawaz F, Huang D, Zheng X, Yang J, Lempicki R, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Okwara N, Wei D, Roby G, David A, Hwang I, Kehrl J, Arthos J, Cicala C, Fauci A The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-beta1 production and FcRL4 expression. Nat Immunol, 2013;14(12):1256-65. 2013 [PMID: 24162774] (Blocking, Human) Blocking Human
Oida T, Weiner HL Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß. J. Immunol., 2012;185(6):3529-35. 2012 [PMID: 20720212] (Western Blot, Human) Western Blot Human
Show All 25 Publications.

Reviews for TGF-beta 1/1.2 Antibody (AF-101-NA) (0)

There are no reviews for TGF-beta 1/1.2 Antibody (AF-101-NA). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TGF-beta 1/1.2 Antibody (AF-101-NA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional TGF-beta 1/1.2 Products

Array AF-101-NA

Blogs on TGF-beta 1/1.2

There are no specific blogs for TGF-beta 1/1.2, but you can read our latest blog posts.
Coronavirus Brochure

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our TGF-beta 1/1.2 Antibody [Unconjugated] and receive a gift card or discount.