TGF-beta 1/1.2 Antibody [Unconjugated]

Images

 
Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

TGF-beta 1/1.2 Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1
Ala270-Ser390
Accession # P01137
Specificity
Detects TGF-beta 1/1.2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with TGF-beta 2 is observed.
Source
N/A
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 5-30 ng/mL
Publications
Read Publications using
AF-101-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGF-beta 1/1.2 Antibody [Unconjugated]

  • TGFb1.2
  • TGF-beta 1/1.2

Background

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 1/1.2 Antibody (AF-101-NA)(25)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Guinea Pig, Mink, Rabbit.

We have publications tested in 8 applications: Blocking, Flow Cytometry, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Blocking
(1)
Flow Cytometry
(2)
IHC
(4)
IHC-Fr
(2)
IHC-P
(1)
Immunoprecipitation
(2)
Neutralization
(10)
Western Blot
(3)
All Applications
Filter By Species
Human
(10)
Mouse
(9)
Rat
(3)
Guinea Pig
(1)
Mink
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using AF-101-NA Applications Species
S Holvoet, M Perrot, N de Groot, G Prioult, T Mikogami, V Verhasselt, S Nutten Oral Tolerance Induction to Newly Introduced Allergen is Favored by a Transforming Growth Factor-beta-Enriched Formula Nutrients, 2019;11(9):. 2019 [PMID: 31540231] (Neutralization, Mink) Neutralization Mink
M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Int J Mol Sci, 2019;20(1):. 2019 [PMID: 30609691] (Neutralization, Human) Neutralization Human
O Miroshnych, WT Chang, JL Dragoo The Use of Platelet-Rich and Platelet-Poor Plasma to Enhance Differentiation of Skeletal Myoblasts: Implications for the Use of Autologous Blood Products for Muscle Regeneration Am J Sports Med, 2017;45(4):945-953. 2017 [PMID: 28027451] (Immunoprecipitation, Human) Immunoprecipitation Human
Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment. Sci Rep, 2015;5(0):8919. 2015 [PMID: 25747469] (IHC, Mouse) IHC Mouse
Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta. Proc Natl Acad Sci U S A, 2014;111(21):E2229-36. 2014 [PMID: 24825889] (Immunoprecipitation, Human) Immunoprecipitation Human
Yeste A, Mascanfroni I, Nadeau M, Burns E, Tukpah A, Santiago A, Wu C, Patel B, Kumar D, Quintana F IL-21 induces IL-22 production in CD4+ T cells. Nat Commun, 2014;5(0):3753. 2014 [PMID: 24796415] (Neutralization, Mouse) Neutralization Mouse
Kang I, Yoon D, Braun K, Wight T Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion. J Biol Chem, 2014;289(22):15393-404. 2014 [PMID: 24719328] (Neutralization, Rat) Neutralization Rat
Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C Long-term gene therapy with thrombospondin 2 inhibits TGF-beta activation, inflammation and angiogenesis in chronic allograft nephropathy. PLoS ONE, 2013;8(12):e83846. 2013 [PMID: 24376766] (IHC, Rat) IHC Rat
Jelicic K, Cimbro R, Nawaz F, Huang D, Zheng X, Yang J, Lempicki R, Pascuccio M, Van Ryk D, Schwing C, Hiatt J, Okwara N, Wei D, Roby G, David A, Hwang I, Kehrl J, Arthos J, Cicala C, Fauci A The HIV-1 envelope protein gp120 impairs B cell proliferation by inducing TGF-beta1 production and FcRL4 expression. Nat Immunol, 2013;14(12):1256-65. 2013 [PMID: 24162774] (Blocking, Human) Blocking Human
Oida T, Weiner HL Overexpression of TGF-ß 1 gene induces cell surface localized glucose-regulated protein 78-associated latency-associated peptide/TGF-ß. J. Immunol., 2012;185(6):3529-35. 2012 [PMID: 20720212] (Western Blot, Human) Western Blot Human
Show All 25 Publications.

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