Testosterone Parameter Assay Kit

• Reactivity: All-Multi
• Applications: ELISA
• Conjugate: Biotin

Testosterone Parameter Assay Kit Summary

• Background: The Parameter Testosterone kit is a 4.5 hour competitive enzyme immunoassay designed to measure Testosterone in cell culture supernates, serum, and plasma.
• Specificity: Measures Testosterone levels in various samples
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Testosterone Parameter Assay Kit

• 17b-Hydroxyandrost-4-ene-3-one
• 17b-Testosterone
• Testosterone

Background

Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Testosterone (KGE010)(56)

Publications using KGE010

• Lautan, O;Chen, SN;Lu, CC;Huang, YH;Chen, HL;Chih, PS;Chen, RJ; Effect-based high-throughput screening of environmental water samples: Focus on liver toxicity pathways and endocrine disruption Journal of hazardous materials 2025-08-05 [PMID: 40773838] (Human)
• Park, YJ;Hwang, SM;Pang, WK;Ryu, DY;Gedda, G;Pang, MG; Bisphenol A-induced testicular immune dysfunction leads to suppressed steroidogenesis and excessive histamine production Ecotoxicology and environmental safety 2025-08-02 [PMID: 40753778] (Mouse)
• Orta Yilmaz, B;Aydin, Y; New insights into the mechanisms underlying 5-hydroxymethylfurfural-induced suppression of testosterone biosynthesis in vivo and in vitro Toxicology and applied pharmacology 2024-12-01 [PMID: 39515622] (Mouse)
• Justin Margret, J;Jain, SK; The association between alpha-1 antitrypsin and B-type natriuretic peptide blood levels in healthy African Americans BMC research notes 2024-11-07 [PMID: 39511686] (Human)
• Ning, S;Armstrong, CM;Xing, E;Leslie, AR;Gao, RY;Sharifi, M;Schaaf, ZA;Lou, W;Han, X;Xu, DH;Yang, R;Cheng, J;Mohammed, S;Mitsiades, N;Liu, C;Lombard, AP;Wu, CY;Cheng, X;Li, PK;Gao, AC; LX1 Targets Androgen Receptor Variants and AKR1C3 to overcome Therapy Resistance in Advanced Prostate Cancer Cancer research 2024-08-01 [PMID: 39088701] (Mouse)
• Yuan, F;Bai, K;Hou, Y;Zou, X;Sun, J; Small Molecule Cocktails Promote Fibroblast-to-Leydig-like Cell Conversion for Hypogonadism Therapy Pharmaceutics 2023-10-13 [PMID: 37896216] (Mouse, Human)
• Ishizaka, T;Horiuchi, K;Kondo, S;Isaji, M;Nakagawa, T;Inoue, M;Rikitake, H;Taguchi, E;Susa, M;Yoda, M;Ono, T;Kozai, Y;Chiba, K; Eribulin mesylate induces bone mass loss by promoting osteoclastic bone resorption in mice Bone reports 2023-06-01 [PMID: 37305428] (Mouse)
• Y Komano, K Fukao, K Shimada, H Naito, Y Ishihara, T Fujii, T Kokubo, H Daida Effects of Ingesting Food Containing Heat-Killed Lactococcus lactis Strain Plasma on Fatigue and Immune-Related Indices after High Training Load: A Randomized, Double-Blind, Placebo-Controlled, and Parallel-Group Study Nutrients, 2023-04-04;15(7):. 2023-04-04 [PMID: 37049594] (Human)
• E Cho, X Che, MJ Ang, S Cheon, J Lee, KS Kim, CH Lee, SY Lee, HY Yang, C Moon, C Park, JY Choi, TH Lee Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration Elife, 2023-02-03;12(0):. 2023-02-03 [PMID: 36735291] (Mouse)
• JH Pérez, E Tolla, VR Bishop, RG Foster, SN Peirson, IC Dunn, SL Meddle, TJ Stevenson Functional inhibition of deep brain non-visual opsins facilitates acute long day induction of reproductive recrudescence in male Japanese quail Hormones and behavior, 2023-01-06;148(0):105298. 2023-01-06 [PMID: 36621293] (Quail)
• J Lu, J Liao, M Qin, H Li, Q Zhang, Y Chen, J Cheng Single-Cell RNAseq Resolve the Potential Effects of LanCL1 Gene in the Mouse Testis Cells, 2022-12-19;11(24):. 2022-12-19 [PMID: 36552898] (Mouse)
• HM Hafez, S Waz, NFG El-Tahawy, MZ Mohamed Agomelatine ameliorates cadmium-induced toxicity through the modification of HMGB-1/TLR-4/NFkappaB pathway Toxicology and Applied Pharmacology, 2022-11-08;457(0):116313. 2022-11-08 [PMID: 36356678] (Rat)
• RM Critelli, F Milosa, A Romanzi, S Lasagni, G Marcelli, L Di Marco, A Pivetti, F Schepis, D Romagnoli, S Mancarella, F Dituri, ML Martinez-C, G Giannelli, E Villa Upregulation of the oestrogen target gene SIX1 is associated with higher growth speed and decreased survival in HCV-positive women with hepatocellular carcinoma Oncology Letters, 2022-09-21;24(5):395. 2022-09-21 [PMID: 36276500] (Human)
• S Yao, X Wei, W Deng, B Wang, J Cai, Y Huang, X Lai, Y Qiu, Y Wang, Y Guan, J Wang Nestin-dependent mitochondria-ER contacts define stem Leydig cell differentiation to attenuate male reproductive ageing Nature Communications, 2022-07-11;13(1):4020. 2022-07-11 [PMID: 35821241] (Mouse)
• WF Lim, M Forouhan, TC Roberts, J Dabney, R Elleringto, AA Speciale, R Manzano, M Lieto, G Sangha, S Banerjee, M Conceição, L Cravo, A Biscans, L Roux, N Pourshafie, C Grunseich, S Duguez, A Khvorova, M Pennuto, CJ Cortes, AR La Spada, KH Fischbeck, MJA Wood, C Rinaldi Gene therapy with AR isoform 2 rescues spinal and bulbar muscular atrophy phenotype by modulating AR transcriptional activity Science Advances, 2021-08-20;7(34):. 2021-08-20 [PMID: 34417184] (Mouse)
• SAE Bashandy, H Ebaid, J Al-Tamimi, OA Ahmed-Fari, EA Omara, IM Alhazza Melatonin Alleviated Potassium Dichromate-Induced Oxidative Stress and Reprotoxicity in Male Rats BioMed Research International, 2021-06-15;2021(0):3565360. 2021-06-15 [PMID: 34222468] (Rat)
• H Zhao, L Song, N Ma, C Liu, Y Dun, Z Zhou, D Yuan, C Zhang The dynamic changes of Nrf2 mediated oxidative stress, DNA damage and base excision repair in testis of rats during aging Experimental gerontology, 2021-06-24;152(0):111460. 2021-06-24 [PMID: 34175407] (Rat)
• MJ Torres, F López-Monc, D Herrera, S Indo, A Lefian, P Llanos, J Tapia, EA Castellón, HR Contreras Endothelin-1 induces changes in the expression levels of steroidogenic enzymes and increases androgen receptor and testosterone production in the PC3 prostate cancer cell line Oncology reports, 2021-06-24;46(2):. 2021-06-24 [PMID: 34165174] (Human)
• CE Metzger, EA Swallow, AJ Stacy, MR Allen Adenine-induced chronic kidney disease induces a similar skeletal phenotype in male and female C57BL/6 mice with more severe deficits in cortical bone properties of male mice PLoS ONE, 2021-04-23;16(4):e0250438. 2021-04-23 [PMID: 33891630] (Mouse)
• M Di Donato, A Zamagni, G Galasso, E Di Zazzo, P Giovannell, MV Barone, M Zanoni, R Gunelli, M Costantini, F Auricchio, A Migliaccio, A Tesei, G Castoria The androgen receptor/filamin A complex as a target in prostate cancer microenvironment Cell Death & Disease, 2021-01-26;12(1):127. 2021-01-26 [PMID: 33500395] (Human)
• H Jin, J Hou, X Meng, T Ma, B Wang, Z Liu, X Sha, J Ding, X Han Microcystin-leucine arginine induced the apoptosis of GnRH neurons by activating the endoplasmic reticulum stress resulting in a decrease of serum testosterone level in mice Ecotoxicology and environmental safety, 2020-12-10;208(0):111748. 2020-12-10 [PMID: 33396074] (Mouse)
• A Wilharm, HC Brigas, I Sandrock, M Ribeiro, T Amado, A Reinhardt, A Demera, L Hoenicke, T Strowig, T Carvalho, I Prinz, JC Ribot Microbiota-dependent expansion of testicular IL-17-producing Vgamma6+ gammadelta T cells upon puberty promotes local tissue immune surveillance Mucosal Immunol, 2020-07-30;0(0):. 2020-07-30 [PMID: 32733025] (Mouse)
• T Velletri, Y Huang, Y Wang, Q Li, M Hu, N Xie, Q Yang, X Chen, Q Chen, P Shou, Y Gan, E Candi, AP Margherita, M Agostini, H Yang, G Melino, Y Shi, Y Wang Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin Cell Death Differ., 2020-07-21;0(0):. 2020-07-21 [PMID: 32694652] (Mouse)
• A Eskandari, M Fashi, A Saeidi, D Boullosa, I Laher, A Ben Abderr, G Jabbour, H Zouhal Resistance Exercise in a Hot Environment Alters Serum Markers in Untrained Males Front Physiol, 2020-06-23;11(0):597. 2020-06-23 [PMID: 32655403] (Human)
• H Zhao, X You, Q Chen, S Yang, Q Ma, Y He, C Liu, Y Dun, J Wu, C Zhang, D Yuan Icariin Improves Age-Related Testicular Dysfunction by Alleviating Sertoli Cell Injury via Upregulation of the ERalpha/Nrf2-Signaling Pathway Front Pharmacol, 2020-05-12;11(0):677. 2020-05-12 [PMID: 32528279] (Rat)
• AL Negrón, G Yu, U Boehm, M Acosta-Mar Targeted Deletion of PTEN in Kisspeptin Cells Results in Brain Region- and Sex-Specific Effects on Kisspeptin Expression and Gonadotropin Release Int J Mol Sci, 2020-03-19;21(6):. 2020-03-19 [PMID: 32204355] (Mouse)
• DS Lee, DW Kang, GW Choi, HG Choi, HY Cho Development of Level A In Vitro-Vivo Correlation for Electrosprayed Microspheres Containing Leuprolide: Physicochemical, Pharmacokinetic, and Pharmacodynamic Evaluation Pharmaceutics, 2020-01-02;12(1):. 2020-01-02 [PMID: 31906491] (Rat)
• Luca De Toni, Diego Guidolin, Vincenzo De Filippis, Daniele Peterle, Maria Santa Rocca, Andrea Di Nisio et al. SHBG141–161 Domain-Peptide Stimulates GPRC6A-Mediated Response in Leydig and beta -Langerhans cell lines Scientific Reports 2019-12-19 [PMID: 31857654] (Mouse)
• Q Zhao, L Gao, Q Liu, Y Cao, Y He, A Hu, W Chen, J Cao, C Hu, L Li, F Tao Impairment of learning and memory of mice offspring at puberty, young adulthood, and adulthood by low-dose Cd exposure during pregnancy and lactation via GABAAR ?5 and ? subunits Ecotoxicol. Environ. Saf., 2018-10-01;166(0):336-344. 2018-10-01 [PMID: 30278395] (Mouse)
• N Fujita, A Takenaka Testosterone represses urinary excretion of the alpha-tocopherol metabolite alpha-carboxymethylhydroxychroman in rats J. Nutr. Biochem., 2018-09-01;62(0):59-64. 2018-09-01 [PMID: 30253278] (Rat)
• Y Bai, C Zhu, M Feng, H Wei, L Li, X Tian, Z Zhao, S Liu, N Ma, X Zhang, R Shi, C Fu, Z Wu, S Zhang Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells Stem Cell Res Ther, 2018-07-18;9(1):200. 2018-07-18 [PMID: 30021628] (Porcine)
• EH Allott, EM Masko, AR Freedland, E Macias, K Pelton, KR Solomon, EA Mostaghel, GV Thomas, SV Pizzo, MR Freeman, SJ Freedland Serum cholesterol levels and tumor growth in a PTEN-null transgenic mouse model of prostate cancer Prostate Cancer Prostatic Dis., 2018-05-23;0(0):. 2018-05-23 [PMID: 29795142] (Mouse)
• DS Lee, SJ Kim, GW Choi, YB Lee, HY Cho Pharmacokinetic-Pharmacodynamic Model for the Testosterone-Suppressive Effect of Leuprolide in Normal and Prostate Cancer Rats Molecules, 2018-04-15;23(4):. 2018-04-15 [PMID: 29662027] (Rat)
• C Skovgaard, D Christians, PM Christense, NW Almquist, M Thomassen, J Bangsbo Effect of speed endurance training and reduced training volume on running economy and single muscle fiber adaptations in trained runners Physiol Rep, 2018-02-01;6(3):. 2018-02-01 [PMID: 29417745] (Human)
• M Dou, K Zhu, Z Fan, Y Zhang, X Chen, X Zhou, X Ding, L Li, Z Gu, M Guo, M Yan, X Deng, P Shen, S Wang Reproductive Hormones and Their Receptors May Affect Lung Cancer Cell. Physiol. Biochem., 2017-11-30;44(4):1425-1434. 2017-11-30 [PMID: 29186712] (Mouse)
• MJ Connolly, N Kerse, T Wilkinson, O Menzies, A Rolleston, YH Chong, JB Broad, SA Moyes, S Jatrana, R Teh Testosterone in advance age: a New Zealand longitudinal cohort study: Life and Living in Advanced Age (Te Pu?waitanga o Ng? Tapuwae Kia Ora Tonu) BMJ Open, 2017-11-12;7(11):e016572. 2017-11-12 [PMID: 29133315] (Human)
• Y Iizuka-His, D Hishikawa, J Sasaki, K Takubo, M Goto, K Nagata, H Nakanishi, H Shindou, T Okamura, C Ito, K Toshimori, T Sasaki, T Shimizu Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis J. Biol. Chem., 2017-06-03;0(0):. 2017-06-03 [PMID: 28578315] (Mouse)
• M Matsuda, M Hirata Phospholipase C-related but Catalytically Inactive Proteins Regulate Ovarian Follicle Development J. Biol. Chem., 2017-03-30;0(0):. 2017-03-30 [PMID: 28360101] (Mouse)
• J Chen, S Meng, H Xu, Z Zhang, X Wu Effects of Microcystis on Hypothalamic-Pituitary-Gonadal-Liver Axis in Nile Tilapia (Oreochromis niloticus) Bull Environ Contam Toxicol, 2017-02-24;0(0):. 2017-02-24 [PMID: 28236009] (Fish - Oreochrmois niloticus (Tilapia))
• N Kim, H Nakamura, H Masaki, K Kumasawa, KI Hirano, T Kimura Effect of lipid metabolism on male fertility Biochem. Biophys. Res. Commun, 2017-02-22;0(0):. 2017-02-22 [PMID: 28235483] (Human)
• ZJ Zang, J Wang, Z Chen, Y Zhang, Y Gao, Z Su, Y Tuo, Y Liao, M Zhang, Q Yuan, C Deng, MH Jiang, AP Xiang Transplantation of CD51(+) stem Leydig cells: A new strategy for the treatment of testosterone deficiency Stem Cells, 2017-03-05;0(0):. 2017-03-05 [PMID: 28090714] (Mouse)
• Jens Bangsbo Effect of increased and maintained frequency of speed endurance training on performance and muscle adaptations in runners J. Appl. Physiol., 2016-11-17;0(0):jap.00537.201. 2016-11-17 [PMID: 27856713] (Human)
• Russi A, Walker-Caulfield M, Ebel M, Brown M Cutting edge: c-Kit signaling differentially regulates type 2 innate lymphoid cell accumulation and susceptibility to central nervous system demyelination in male and female SJL mice. J Immunol, 2015-05-13;194(12):5609-13. 2015-05-13 [PMID: 25972476] (Mouse)
• Fan , Yong, Liu , Yue, Xue , Ke, Gu , Guobao, Fan , Weimin, Xu , Yali, Ding , Zhide Diet-induced obesity in male C57BL/6 mice decreases fertility as a consequence of disrupted blood-testis barrier. PLoS ONE, 2015-04-17;10(4):e0120775. 2015-04-17 [PMID: 25886196] (Mouse)
• Patel , Divya, Knowell , Ashley E, Korang-Yeboah , Maxwell, Sharma , Pankaj, Joshi , Jugal, Glymph , Shanora, Chinaranagari , Swathi, Nagappan , Peri, Palaniappan , Ravi, Bowen , Nathan J, Chaudhary , Jaideep Inhibitor of differentiation 4 (ID4) inactivation promotes de novo steroidogenesis and castration-resistant prostate cancer. Mol Endocrinol, 2014-06-12;28(8):1239-53. 2014-06-12 [PMID: 24921661] (Human)
• Lancha A, Rodriguez A, Catalan V, Becerril S, Sainz N, Ramirez B, Burrell M, Salvador J, Fruhbeck G, Gomez-Ambrosi J Osteopontin deletion prevents the development of obesity and hepatic steatosis via impaired adipose tissue matrix remodeling and reduced inflammation and fibrosis in adipose tissue and liver in mice. PLoS ONE, 2014-05-28;9(5):e98398. 2014-05-28 [PMID: 24871103] (Mouse)
• Hu , Yang, Wang , Ruoyu, Xiang , Zou, Qian , Weiping, Han , Xiaodong, Li , Dongmei Antagonistic effects of a mixture of low-dose nonylphenol and di-n-butyl phthalate (monobutyl phthalate) on the Sertoli cells and serum reproductive hormones in prepubertal male rats in vitro and in vivo. PLoS ONE, 2014-03-27;9(3):e93425. 2014-03-27 [PMID: 24676355] (Rat)
• Zhu T, Griffith J, Qin L, Hung V, Fong T, Au S, Li M, Lam Y, Wong C, Kwok A, Leung P, Li E, Tam L Alterations of bone density, microstructure, and strength of the distal radius in male patients with rheumatoid arthritis: a case-control study with HR-pQCT. J Bone Miner Res, 2014-09-01;29(9):2118-29. 2014-09-01 [PMID: 24644043] (Human)
• Xue , Kai, Kim , Ji Young, Liu , Jia-yin, Tsang , Benjamin Insulin-like 3-induced rat preantral follicular growth is mediated by growth differentiation factor 9. Endocrinology, 2013-12-20;155(1):156-67. 2013-12-20 [PMID: 24169563] (Rat)
• Fowler JA, Lwin ST, Drake MT Host-derived adiponectin is tumor-suppressive and a novel therapeutic target for multiple myeloma and the associated bone disease. Blood, 2011-09-08;118(22):5872-82. 2011-09-08 [PMID: 21908434] (Human)
• Betts JA, Beelen M, Stokes KA, Saris W HM, van Loon L JC Endocrine responses during overnight recovery from exercise: impact of nutrition and relationships with muscle protein synthesis. Int J Sport Nutr Exerc Metab, 2011-08-10;21(5):398-409. 2011-08-10 [PMID: 21832304] (Human)
• Oatley MJ, Kaucher AV, Racicot KE Inhibitor of DNA binding 4 is expressed selectively by single spermatogonia in the male germline and regulates the self-renewal of spermatogonial stem cells in mice. Biol. Reprod., 2011-05-04;85(2):347-56. 2011-05-04 [PMID: 21543770] (Mouse)
• Hong H, Nadesan P, Poon R, Alman BA Testosterone regulates cell proliferation in aggressive fibromatosis (desmoid tumour). Br. J. Cancer, 2011-04-05;104(9):1452-8. 2011-04-05 [PMID: 21468052] (Mouse)
• Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders. PLoS ONE, 2010-12-20;5(12):e15606. 2010-12-20 [PMID: 21187972] (Human)
• Jiao Q, Pruznak AM, Huber D, Vary TC, Lang CH Castration Differentially Alters Basal and Leucine-Stimulated Tissue Protein Synthesis in Skeletal Muscle and Adipose Tissue. Am. J. Physiol. Endocrinol. Metab., 2009-09-15;0(0):. 2009-09-15 [PMID: 19755668] (Rat)
• Zhang W, Zhu J, Efferson CL, Ware C, Tammam J, Angagaw M, Laskey J, Bettano KA, Kasibhatla S, Reilly JF, Sur C, Majumder PK Inhibition of tumor growth progression by antiandrogens and mTOR inhibitor in a Pten-deficient mouse model of prostate cancer. Cancer Res., 2009-09-08;69(18):7466-72. 2009-09-08 [PMID: 19738074] (Mouse)

FAQs for Testosterone (KGE010). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.