![Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Testosterone monoclonal antibody (4E1G2).](http://images.novusbio.com/fullsize/Testosterone-Antibody-4E1G2-Immunohistochemistry-Paraffin-NBP2-45187-img0001.jpg "Immunohistochemistry-Paraffin: Testosterone Antibody (4E1G2) [NBP2-45187] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Testosterone monoclonal antibody (4E1G2).")
| Reactivity | All-NASpecies Glossary |
| Applications | ELISA, Func, IHC |
| Clone | 4E1G2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11603) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Testosterone 3 CMO conjugated to BSA |
| Localization | Secreted and cytoplasmic |
| Specificity | This monoclonal antibody is highly specific to testosterone. Its affinity constant for testosterone is ~1010M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: For coating, order Ab without BSA. Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 288.42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")