Teprotumumab Antibody (975547) [Unconjugated]

MCF-7 human cell line was stained with Human anti-Human IGFLR-1 (Teprotumumab) Monoclonal Antibody (Catalog # MAB9718) then Anti-Teprotumumab (Anti-Idiotype) (Catalog # MAB96341, filled histogram) or Mouse IgG2A isotype control antibody(Catalog # MAB003, open histogram) followed by PE-conjugated anti-mouse IgG secondary antibody (Catalog # F0102B).

In a functional flow cytometry test, Biotinylated Anti-IGFLR-1 (Teprotumumab) (Catalog # FAB9718B) binds to MCF-7 human cell line (black dotted line). Binding is completely blocked (orange histogram) by 0.25 μg/mL of Anti-Teprotumumab (Anti-idiotype) Monoclonal Antibody (Catalog# MAB96341), but not by Normal Mouse IgG2A (Catalog # MAB003) (blue line). Cells were stained with APC-conjugated Streptavidin (Catalog # F0050).

• Reactivity: All-Multi
• Applications: Flow, B/N
• Clone: 975547
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Teprotumumab Antibody (975547) [Unconjugated] Summary

• Immunogen: Full-length Teprotumumab
• Specificity: Detects Teprotumumab in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Binding Inhibition 2.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Teprotumumab Antibody (975547) [Unconjugated]

• Teprotumumab

Background

The anti-Teprotumumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Teprotumumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.