Teprotumumab Antibody (975505) [Unconjugated]

Specificity of Anti-Teprotumumab (Anti-Idiotype) Antibody in Direct ELISA A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Teprotumumab (Anti-Idiotype) Antibody (Catalog # MAB9634, 333 ng/mL) detected Teprotumumab. No cross-reactivity with Cetuximab, Adalimumab, Rituximab, Trastuzumab, Brentuximab, Gemtuzumab, Ibritumomab, Tositumomab, Solanezumab and Aducanumab was observed.

Western blot of Centuximab, Rituximab, Trastuzumab, and Teprotumumab. PVDF membrane was probed with 2 µg/mL of Anti- Teprotumumab (Anti-Idiotype) Monoclonal Antibody (Catalog # MAB9634) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Teprotumumab at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: All-Multi
• Applications: WB, ELISA
• Clone: 975505
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Teprotumumab Antibody (975505) [Unconjugated] Summary

• Immunogen: Full-length Teprotumumab
• Specificity: Detects Teprotumumab in Western blots and direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Teprotumumab Antibody (975505) [Unconjugated]

• Teprotumumab

Background

The anti-Teprotumumab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Teprotumumab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.