TcBuster Transposase Antibody (1077035) [Unconjugated]

Western Blot shows lysates of HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation. PVDF membrane was probed with 1 µg/ml of Mouse Anti-TcBuster-M Transposase Monoclonal Antibody (Catalog # MAB11511) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TcBuster-M Transposase at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western shows lysates HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1µg TcBuster-M Transposase after 1 day post-electroporation, loaded at 0.5 mg/ml. A specific band was detected for TcBuster-M Transposase at approximately 71 kDa (as indicated) using 20 µg/mL of Mouse Anti-TcBuster-M Transposase Monoclonal Antibody (Catalog # MAB11511). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

• Reactivity: All-Multi
• Applications: WB, Simple Western
• Clone: 1077035
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TcBuster Transposase Antibody (1077035) [Unconjugated] Summary

• Immunogen: Synthetic Peptide
• Specificity: Detects TcBuster-M Transposase in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TcBuster Transposase Antibody (1077035) [Unconjugated]

• GLEAN_06077
• TcasGA2_TC006077
• TcBuster Transposase
• Transposase

Background

TcBuster is a non-viral gene delivery system that enables stable gene transfer in most cell types. The TcBuster system belongs to the hAT-family of DNA transposons and is derived from the red flour beetle Tribolium castaneum (1). It consists of the TcBuster transposase mRNA and DNA transposon encoding multicistronic cargos for delivery. This antibody binds to the TcBuster-M transposase enzyme, a hyperactive version of TcBuster and carriers out the cut-and-paste reactions for efficient delivery of DNA cargos (2). TcBuster is used in a wide variety of genome engineering applications for both research and commercial purposes. These include, but are not limited to, immune cell therapies (T, NK cells), stem cell therapies for regenerative medicine (iPSCs), and bioproduction of biologics, such as monoclonal antibodies. The TcBuster system is available for purchase from Bio-Techne. For more information, please visit https://www.bio-techne.com/services/gene-engineering-services-tcbuster.

1. Woodard, L.E. et al. (2012) PLOS One DOI: 10.1371/journal.pone.0042666.
2. Skeate, J.G. et al. (2024) Molecular Therapy DOI: 10.1016/j.ymthe.2024.04.024.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.