Tat-Beclin 1 L11S Peptide - Scrambled Control

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Western Blot: Tat-Beclin 1 L11S Autophagy Inducing Peptide - Inactive Form [NBP2-49887] - WB analysis of lysates from HeLa cells that were left untreated (blank) or were treated with 10-20 uM each of Tat-D11, Tat-L11, ...read more
Immunocytochemistry/ Immunofluorescence: Tat-Beclin 1 L11S Autophagy Inducing Peptide - Inactive Form [NBP2-49887] - HeLa GFP-LC3B cells were treated with Tat-D11, Tat-L11, Tat-Beclin 1 or Tat-L11S for 1.5 hours. ...read more
In vivo assay: Tat-Beclin 1 L11S Peptide - Scrambled Control [NBP2-49887] - In vivo dose study in mouse kidneys as control for tat-beclin D11 autophagy inducing peptide. Well tolerated by mice at dose of 1mg/kg for 2 ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications Func, In vitro, In vivo

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Tat-Beclin 1 L11S Peptide - Scrambled Control Summary

Description
Tat-L11S [NBP2-49887]: inactive/scrambled control peptides derived from Tat-L11. These peptides are recommended as a negative control. The exact sequence of Tat-Beclin 1 L11S is YGRKKRRQRRRGGNWAWHDFVHIT (Bio-Techne's exclusive patent license: US Patent 8,802,633)
Background
Cell-penetrating autophagy inducing peptides engineered in 2013 were demonstrated to induce autophagy through interaction with the autophagy suppressor GAPR-1/GLIPR2 (Nature, 2013; PMID 23364696). These Tat-Beclin 1 peptides were comprised of AA 267-284 of the autophagy inducer Beclin 1 (18 amino acids), a diglycine linker, and 11 amino acids of the HIV Tat protein transduction domain. Tat-B...eclin 1 peptides were re-engineered to remove 7 AA from the beclin 1 domain. These shorter peptides, Tat-D11 [NBP2-49888] and Tat-L11 [NBP2-49886], demonstrate enhanced autophagy inducing function over the original Tat-Beclin 1 peptides and the inactive/scrambled control peptide Tat-L11S [NBP2-49887]. Tat-D11 and Tat-L11 are potent autophagy inducers which function both in vitro and in vivo to specifically induce autophagy while Tat-L11S is useful as a negative control. Tat-D11 and Tat-L11 peptides are comprised of 11 amino acids of the autophagy-inducing region of beclin 1 fused to the HIV Tat protein. Both Tat-D11 and Tat-L11 peptides function by binding the negative regulator of autophagy GAPR-1/GLIPR2. Upon peptide binding, beclin 1 bound to GARP-1 is released, resulting in beclin 1 mediated autophagosome formation and autophagy induction. Data indicate Tat-D11 is more potent than both Tat-L11 and Tat-Beclin 1 in vivo. In addition, initial data derived from HeLa cells also suggests Tat-D11 is more potent than Tat-L11 in vitro. However, ongoing experiments indicate Tat-L11 may be more potent than Tat-D11 in select cell lines.
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Protein/Peptide Type
Peptide

Applications/Dilutions

Reviewed Applications
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NBP2-49887 in the following application:

Publications
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NBP2-49887 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Buffer
This product is supplied lyophilized. Purity is >= to 97% (HPLC)
Reconstitution Instructions
Reconstitute with DMSO or water to desired concetration.

Alternate Names for Tat-Beclin 1 L11S Peptide - Scrambled Control

  • Autophagy Inducing peptide
  • Tat-Beclin 1 peptide
  • Tat-Beclin 1
  • Tat-Beclin peptide
  • Tat-Beclin
  • Tat-L11S

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Publications for Tat-Beclin 1 Peptide (NBP2-49887)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Func, WB, in vivo.


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Showing Publications 1 - 3 of 3.
Publications using NBP2-49887 Applications Species
Wong M, Ganapathy AS, Suchanec E et al. Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1 Am. J. Physiol., Cell Physiol. Mar 20 2019 [PMID: 30892937] (Func) Func
Sharif T, Martell E, Dai C et al. HDAC6 differentially regulates autophagy in stem-like versus differentiated cancer cells. Autophagy. Nov 16 2018 [PMID: 30444165] (WB, Human) WB Human
Shoji-Kawata S, Sumpter R, Leveno M et al. Identification of a candidate therapeutic autophagy-inducing peptide. Nature. 2013 Feb 14 [PMID: 23364696] (in vivo)

Details:
Tat-beclin 1 (L-amino acid) / Tat-Beclin 1 L11 (NBP2-49886) and Tat–beclin 1 (D-amino acid)/Tat-Beclin 1 D11, Retroinverso form (NBP2-49888) along with the control peptide Tat-scrambled (L-amino acid)/Tat-Beclin 1 L11S Peptide, Scrambled Control (NBP2-49887) were tested in-vivo for the induction of autophagy. 6-week-old GFP-LC3 transgenic mice, and normal or CHIKV and WNV Egypt strain virus infected 5-day-old GFP-LC3 mice were injected intra-peritoneally (i.p.) with these Tat-beclin derivative peptides at a dose of 20 mg/Kg body weight (5.3uM/Kg). Brain tissues were analyzed using IHC-Frozen and Western blot analysis for measuring cell death (TUNEL Assay) and p62 expression respectively. Toxicity of these peptides was assessed in 6-day-old C57BL/6J mice via daily injections of Tat-scrambled (L-amino acid, Tat-Beclin 1 L11S) or Tat-beclin 1 (L-amino acid, Tat-Beclin 1 L11) at 15 mg kg-1 and Tat–beclin 1 (D-amino acid, Tat-Beclin 1 D11) at a dose of 20 mg/Kg body weight for 2 weeks (Figure 4).
in vivo

Review for Tat-Beclin 1 Peptide (NBP2-49887) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-49887:
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Immunocytochemistry/Immunofluorescence Tat-Beclin 1 NBP2-49887
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reviewed by:
Nicole Brown
ICC/IF 01/07/2019
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Lot1435396

Comments

Comments*See also review for tat-beclin D11 autophagy inducing peptide

Used for in vivo dose study in mouse kidneys as control for tat-beclin D11 autophagy inducing peptide.
Well tolerated by mice at dose of 1mg/kg for 2 days IP. Soluble in PBS.

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Nicole Brown
01/07/2019
Application: ICC/IF
Species: