Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form

Images

 
Western Blot: Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form [NBP2-49888] - WB analysis of lysates from HeLa cells that were left untreated (blank) or were treated with 10-20 uM each of Tat-D11, ...read more
Immunocytochemistry/ Immunofluorescence: Tat-Beclin 1 D11 Autophagy Inducing Peptide [NBP2-49888] - HeLa GFP-LC3B cells were treated with Tat-D11, Tat-L11, Tat-Beclin 1 or Tat-L11S for 1.5 hours. Thereafter, the cells ...read more

Product Details

Summary
Reactivity NASpecies Glossary
Applications Func, In vitro, In vivo

Order Details

Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form Summary

Description
Tat-D11 [NBP2-49888]: peptides comprising 11 amino acids derived from Beclin 1 linked to the HIV Tat protein with a diglycine linker. These peptides are in the retero-inverso D-configuration. The amino acid sequence of Tat-Beclin 1 D11 is RRRQRRKKRGYGGDHWIHFTANWV (Bio-Techne's exclusive patent license: US Patent 8,802,633).
Background
Cell-penetrating autophagy inducing peptides engineered in 2013 were demonstrated to induce autophagy through interaction with the autophagy suppressor GAPR-1/GLIPR2 (Nature, 2013; PMID 23364696). These Tat-Beclin 1 peptides were comprised of AA 267-284 of the autophagy inducer Beclin 1 (18 amino acids), a diglycine linker, and 11 amino acids of the HIV Tat protein transduction domain. Tat-B...eclin 1 peptides were re-engineered to remove 7 AA from the beclin 1 domain. These shorter peptides, Tat-D11 [NBP2-49888] and Tat-L11 [NBP2-49886], demonstrate enhanced autophagy inducing function over the original Tat-Beclin 1 peptides and the scrambled control peptide Tat-L11S [NBP2-49887]. Tat-D11 and Tat-L11 are potent autophagy inducers which function both in vitro and in vivo to specifically induce autophagy. Tat-D11 and Tat-L11 peptides are comprised of 11 amino acids of the autophagy-inducing region of beclin 1 fused to the HIV Tat protein. Both Tat-D11 and Tat-L11 peptides function by binding the negative regulator of autophagy GAPR-1/GLIPR2. Upon peptide binding, beclin 1 bound to GARP-1 is released, resulting in beclin 1 mediated autophagosome formation and autophagy induction. Data indicate Tat-D11 is more potent than both Tat-L11 and Tat-Beclin 1 in vivo. In addition, initial data derived from HeLa cells also suggests Tat-D11 is more potent than Tat-L11 in vitro. However, ongoing experiments indicate Tat-L11 may be more potent than Tat-D11 in select cell lines.
Show More
Protein/Peptide Type
Autophagy Inducing Peptide

Packaging, Storage & Formulations

Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Buffer
This product is supplied lyophilized. Purity is >= to 97% (HPLC)
Reconstitution Instructions
Reconstitute with DMSO or water to desired concetration. Note:- D11 should NOT be reconstituted at a concentration greater than 5 mM.

Notes

Note: Tat-L11 and Tat-D11, are exclusively available from Novus Biologicals (Bio-Techne's exclusive patent license: US Patent 8,802,633).

Alternate Names for Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form

  • Autophagy Inducing peptide
  • Tat-Beclin 1 peptide
  • Tat-Beclin 1
  • Tat-Beclin peptide
  • Tat-Beclin
  • Tat-D11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Publications for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888) (0)

There are no publications for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888) (0)

There are no reviews for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

View specific protocols for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Tat-Beclin 1 Products

Tat-Beclin 1 NBP2-49888

Research Areas for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888)

Find related products by research area.

Blogs on Tat-Beclin 1

There are no specific blogs for Tat-Beclin 1, but you can read our latest blog posts.

Contact Information

Product PDFs

Review this Product

Be the first to review our Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form and receive a gift card or discount.

Customers Who Bought This Also Bought