Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form

Images

 
Immunocytochemistry/ Immunofluorescence: Tat-Beclin 1 D11 Autophagy Inducing Peptide [NBP2-49888] - HeLa GFP-LC3B cells were treated with Tat-D11, Tat-L11, Tat-Beclin 1 or Tat-L11S for 1.5 hours. Thereafter, the cells ...read more
In vitro assay: Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form [NBP2-49888] - Analysis of lysates from HeLa cells that were left untreated (blank) or were treated with 10-20 uM each of Tat-D11, Tat-L11, ...read more
In vivo assay: Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form [NBP2-49888] - In vivo dose study in 10wk old C57BL/6J mice. Either 1mg/kg or 10mg/kg IP once daily was administered for 2 days, mice were ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func, In vitro, In vivo
Concentration
LYOPH

Order Details

Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form Summary

Description
Tat-D11 [NBP2-49888]: peptides comprising 11 amino acids derived from Beclin 1 linked to the HIV Tat protein with a diglycine linker. These peptides are in the retero-inverso D-configuration. The amino acid sequence of Tat-Beclin 1 D11 is RRRQRRKKRGYGGDHWIHFTANWV (Bio-Techne's exclusive patent license: US Patent 8,802,633).
Background
Cell-penetrating autophagy inducing peptides engineered in 2013 were demonstrated to induce autophagy through interaction with the autophagy suppressor GAPR-1/GLIPR2 (Nature, 2013; PMID 23364696). These Tat-Beclin 1 peptides were comprised of AA 267-284 of the autophagy inducer Beclin 1 (18 amino acids), a diglycine linker, and 11 amino acids of the HIV Tat protein transduction domain. Tat-B...eclin 1 peptides were re-engineered to remove 7 AA from the beclin 1 domain. These shorter peptides, Tat-D11 [NBP2-49888] and Tat-L11 [NBP2-49886], demonstrate enhanced autophagy inducing function over the original Tat-Beclin 1 peptides and the scrambled control peptide Tat-L11S [NBP2-49887]. Tat-D11 and Tat-L11 are potent autophagy inducers which function both in vitro and in vivo to specifically induce autophagy. Tat-D11 and Tat-L11 peptides are comprised of 11 amino acids of the autophagy-inducing region of beclin 1 fused to the HIV Tat protein. Both Tat-D11 and Tat-L11 peptides function by binding the negative regulator of autophagy GAPR-1/GLIPR2. Upon peptide binding, beclin 1 bound to GARP-1 is released, resulting in beclin 1 mediated autophagosome formation and autophagy induction. Data indicate Tat-D11 is more potent than both Tat-L11 and Tat-Beclin 1 in vivo. In addition, initial data derived from HeLa cells also suggests Tat-D11 is more potent than Tat-L11 in vitro. However, ongoing experiments indicate Tat-L11 may be more potent than Tat-D11 in select cell lines.
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Protein/Peptide Type
Autophagy Inducing Peptide

Applications/Dilutions

Theoretical MW
3.08 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
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NBP2-49888 in the following application:

Publications
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NBP2-49888 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Buffer
This product is supplied lyophilized. Purity is >= to 97% (HPLC)
Concentration
LYOPH
Reconstitution Instructions
Reconstitute with DMSO or water to desired concetration. Note:- D11 should NOT be reconstituted at a concentration greater than 5 mM.

Notes

Note: Tat-L11 and Tat-D11, are exclusively available from Novus Biologicals (Bio-Techne's exclusive patent license: US Patent 8,802,633).

Alternate Names for Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form

  • Autophagy Inducing peptide
  • Tat-Beclin 1 peptide
  • Tat-Beclin 1
  • Tat-Beclin peptide
  • Tat-Beclin
  • Tat-D11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Publications for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Func, In vitro, In vivo, in vivo.


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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP2-49888 Applications Species
Pavel M, Park SJ, Frake RA et al. alpha-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation Nature communications Mar 17 2021 [PMID: 33731717]
Mathew B, Chennakesavalu M, Sharma M et al. Autophagy and post-ischemic conditioning in retinal ischemia Autophagy May 26 2020 [PMID: 32452260] (In vitro, Rat) In vitro Rat
Iaconis D, Crina C, Brillante S et al. The HOPS Complex Subunit VPS39 controls ciliogenesis through autophagy Hum. Mol. Genet. Feb 20 2020 [PMID: 32077937] (Human) Human
Forte M, Bianchi F, Cotugno M et al. Pharmacological restoration of autophagy reduces hypertension-related stroke occurrence Autophagy. Nov 12 2019 [PMID: 31679456]
Watanabe R, Sasaki S, Kimura N Activation of autophagy in early neonatal mice increases primordial follicle number and improves lifelong fertility Biol. Reprod. 2019 Sep 30 [PMID: 31566206]
Wong M, Ganapathy AS, Suchanec E et al. Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1 Am. J. Physiol., Cell Physiol. Mar 20 2019 [PMID: 30892937] (Func) Func
Vega-Rubin-de-Celis S, Zou Z, Fernandez AF et al. Increased autophagy blocks HER2-mediated breast tumorigenesis Proc Natl Acad Sci U S A 2018 Apr 17 [PMID: 29610308] (In vivo, Human) In vivo Human
Bartolomeo R, Cinque L, De Leonibus C mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017 Oct 2 [PMID: 28872463] (In vivo, Mouse) In vivo Mouse
Peraro L, Zou Z, Makwana KM et al. Diversity-Oriented Stapling Yields Intrinsically Cell-Penetrant Inducers of Autophagy J Am Chem Soc. 2017 Jun 14 [PMID: 28414223] (In vivo, Human) In vivo Human
Franco LH, Nair VR, Scharn CR et al. The Ubiquitin Ligase Smurf1 Functions in Selective Autophagy of Mycobacterium tuberculosis and Anti-tuberculous Host Defense. Cell Host Microbe 2017 Jan 11 [PMID: 28017659] (In vivo, Mouse) In vivo Mouse
Show All 14 Publications.

Review for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49888) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP2-49888:
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 Tat-Beclin 1 NBP2-49888
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5
reviewed by:
Nicole Brown
01/07/2019
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Summary

Lot1455072
CommentsIncreased basal expression of LC3-II on immunoblot as expected in the kidneys at a dose of 1mg/kg.Soluble in PBS.

Comments

CommentsI used this peptide for an in vivo dose study in 10wk old C57BL/6J mice. I administered either 1mg/kg or 10mg/kg IP once daily for 2 days, sacrificed the mice, and prepared the kidneys for western blot analysis (image shown). Additionally, the lysosomal inhibitor bafilomycin A1 was used to provide a measurement of autophagic flux.
*vehicle is scrambled tat-beclin (NBP2-49887-5mg)

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Nicole Brown
01/07/2019
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