Tat-Beclin 1 L11 Autophagy Inducing Peptide

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Western Blot: Tat-Beclin 1 L11 Autophagy Inducing Peptide [NBP2-49886] - WB analysis of lysates from HeLa cells that were left untreated (blank) or were treated with 10-20 uM each of Tat-D11, Tat-L11, Tat-Beclin 1 or ...read more
Immunocytochemistry/ Immunofluorescence: Tat-Beclin 1 L11 Autophagy Inducing Peptide [NBP2-49886] - HeLa GFP-LC3B cells were treated with Tat-D11, Tat-L11, Tat-Beclin 1 or Tat-L11S for 1.5 hours. Thereafter, the cells ...read more

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func, In vitro, In vivo

Order Details

Tat-Beclin 1 L11 Autophagy Inducing Peptide Summary

Description
Tat-L11 [NBP2-49886]: peptides comprising 11 amino acids derived from Beclin 1 linked to the HIV Tat protein with a diglycine linker. These peptides are in the naturally occurring L-configuration. The exact sequence of Tat-Beclin 1 L11 is YGRKKRRQRRRGGVWNATFHIWHD (Bio-Techne's exclusive patent license: US Patent 8,802,633).
Background
Cell-penetrating autophagy inducing peptides engineered in 2013 were demonstrated to induce autophagy through interaction with the autophagy suppressor GAPR-1/GLIPR2 (Nature, 2013; PMID 23364696). These Tat-Beclin 1 peptides were comprised of AA 267-284 of the autophagy inducer Beclin 1 (18 amino acids), a diglycine linker, and 11 amino acids of the HIV Tat protein transduction domain. Tat-B...eclin 1 peptides were re-engineered to remove 7 AA from the beclin 1 domain. These shorter peptides, Tat-D11 [NBP2-49888] and Tat-L11 [NBP2-49886], demonstrate enhanced autophagy inducing function over the original Tat-Beclin 1 peptides and the scrambled control peptide Tat-L11S [NBP2-49887]. Tat-D11 and Tat-L11 are potent autophagy inducers which function both in vitro and in vivo to specifically induce autophagy. Tat-D11 and Tat-L11 peptides are comprised of 11 amino acids of the autophagy-inducing region of beclin 1 fused to the HIV Tat protein. Both Tat-D11 and Tat-L11 peptides function by binding the negative regulator of autophagy GAPR-1/GLIPR2. Upon peptide binding, beclin 1 bound to GARP-1 is released, resulting in beclin 1 mediated autophagosome formation and autophagy induction. Data indicate Tat-D11 is more potent than both Tat-L11 and Tat-Beclin 1 in vivo. In addition, initial data derived from HeLa cells also suggests Tat-D11 is more potent than Tat-L11 in vitro. However, ongoing experiments indicate Tat-L11 may be more potent than Tat-D11 in select cell lines.
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Protein/Peptide Type
Autophagy Inducing Peptide

Applications/Dilutions

Theoretical MW
3.08 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-49886 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C in powder form. Store at -80C once reconstituted.
Buffer
This product is supplied lyophilized. Purity is >= to 97% (HPLC)
Reconstitution Instructions
Reconstitute with DMSO or water to desired concentration.

Notes

Note: Tat-L11 and Tat-D11, are exclusively available from Novus Biologicals (Bio-Techne's exclusive patent license: US Patent 8,802,633).

Alternate Names for Tat-Beclin 1 L11 Autophagy Inducing Peptide

  • Autophagy Inducing peptide
  • Tat-Beclin 1 peptide
  • Tat-Beclin 1
  • Tat-Beclin peptide
  • Tat-Beclin
  • Tat-L11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Publications for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49886)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: In vivo, in vivo.


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In vivo
(5)
in vivo
(1)
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Human
(2)
Mouse
(3)
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Showing Publications 1 - 6 of 6.
Publications using NBP2-49886 Applications Species
Vega-Rubin-de-Celis S, Zou Z, Fernandez AF et al. Increased autophagy blocks HER2-mediated breast tumorigenesis Proc Natl Acad Sci U S A 2019 Apr 17 [PMID: 29610308] (In vivo, Human) In vivo Human
Bartolomeo R, Cinque L, De Leonibus C mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017 Oct 2 [PMID: 28872463] (In vivo, Mouse) In vivo Mouse
Peraro L, Zou Z, Makwana KM et al. Diversity-Oriented Stapling Yields Intrinsically Cell-Penetrant Inducers of Autophagy J Am Chem Soc. 2017 Jun 14 [PMID: 28414223] (In vivo, Human) In vivo Human
Franco LH, Nair VR, Scharn CR et al. The Ubiquitin Ligase Smurf1 Functions in Selective Autophagy of Mycobacterium tuberculosis and Anti-tuberculous Host Defense. Cell Host Microbe 2017 Jan 11 [PMID: 28017659] (In vivo, Mouse) In vivo Mouse
Pietrocola F, Pol J Vacchelli E et al. Caloric Restriction Mimetics Enhance Anticancer Immunosurveillance Cancer Cell. 2016 Jul 11 [PMID: 27411589] (In vivo, Mouse) In vivo Mouse
Shoji-Kawata S, Sumpter R, Leveno M et al. Identification of a candidate therapeutic autophagy-inducing peptide. Nature. 2013 Feb 14 [PMID: 23364696] (in vivo)

Details:
Tat-beclin 1 (L-amino acid) / Tat-Beclin 1 L11 (NBP2-49886) and Tat–beclin 1 (D-amino acid)/Tat-Beclin 1 D11, Retroinverso form (NBP2-49888) along with the control peptide Tat-scrambled (L-amino acid)/Tat-Beclin 1 L11S Peptide, Scrambled Control (NBP2-49887) were tested in-vivo for the induction of autophagy. 6-week-old GFP-LC3 transgenic mice, and normal or CHIKV and WNV Egypt strain virus infected 5-day-old GFP-LC3 mice were injected intra-peritoneally (i.p.) with these Tat-beclin derivative peptides at a dose of 20 mg/Kg body weight (5.3uM/Kg). Brain tissues were analyzed using IHC-Frozen and Western blot analysis for measuring cell death (TUNEL Assay) and p62 expression respectively. Toxicity of these peptides was assessed in 6-day-old C57BL/6J mice via daily injections of Tat-scrambled (L-amino acid, Tat-Beclin 1 L11S) or Tat-beclin 1 (L-amino acid, Tat-Beclin 1 L11) at 15 mg kg-1 and Tat–beclin 1 (D-amino acid, Tat-Beclin 1 D11) at a dose of 20 mg/Kg body weight for 2 weeks (Figure 4).
in vivo

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