Tat-Beclin 1 L11 Autophagy Inducing Peptide


Western Blot: Tat-Beclin 1 L11 Autophagy Inducing Peptide [NBP2-49886] - WB analysis of lysates from HeLa cells that were left untreated (blank) or were treated with 10-20 uM each of Tat-D11, Tat-L11, Tat-Beclin 1 or ...read more
Immunocytochemistry/ Immunofluorescence: Tat-Beclin 1 L11 Autophagy Inducing Peptide [NBP2-49886] - HeLa GFP-LC3B cells were treated with Tat-D11, Tat-L11, Tat-Beclin 1 or Tat-L11S for 1.5 hours. Thereafter, the cells ...read more

Product Details

Reactivity All-NASpecies Glossary
Applications Func, In vitro, In vivo

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Tat-Beclin 1 L11 Autophagy Inducing Peptide Summary

Tat-L11 [NBP2-49886]: peptides comprising 11 amino acids derived from Beclin 1 linked to the HIV Tat protein with a diglycine linker. These peptides are in the naturally occurring L-configuration. The exact sequence of Tat-Beclin 1 L11 is YGRKKRRQRRRGGVWNATFHIWHD (Bio-Techne's exclusive patent license: US Patent 8,802,633).
Cell-penetrating autophagy inducing peptides engineered in 2013 were demonstrated to induce autophagy through interaction with the autophagy suppressor GAPR-1/GLIPR2 (Nature, 2013; PMID 23364696). These Tat-Beclin 1 peptides were comprised of AA 267-284 of the autophagy inducer Beclin 1 (18 amino acids), a diglycine linker, and 11 amino acids of the HIV Tat protein transduction domain. Tat-B...eclin 1 peptides were re-engineered to remove 7 AA from the beclin 1 domain. These shorter peptides, Tat-D11 [NBP2-49888] and Tat-L11 [NBP2-49886], demonstrate enhanced autophagy inducing function over the original Tat-Beclin 1 peptides and the scrambled control peptide Tat-L11S [NBP2-49887]. Tat-D11 and Tat-L11 are potent autophagy inducers which function both in vitro and in vivo to specifically induce autophagy. Tat-D11 and Tat-L11 peptides are comprised of 11 amino acids of the autophagy-inducing region of beclin 1 fused to the HIV Tat protein. Both Tat-D11 and Tat-L11 peptides function by binding the negative regulator of autophagy GAPR-1/GLIPR2. Upon peptide binding, beclin 1 bound to GARP-1 is released, resulting in beclin 1 mediated autophagosome formation and autophagy induction. Data indicate Tat-D11 is more potent than both Tat-L11 and Tat-Beclin 1 in vivo. In addition, initial data derived from HeLa cells also suggests Tat-D11 is more potent than Tat-L11 in vitro. However, ongoing experiments indicate Tat-L11 may be more potent than Tat-D11 in select cell lines.
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Protein/Peptide Type
Autophagy Inducing Peptide


Theoretical MW
3.08 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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NBP2-49886 in the following applications:

Packaging, Storage & Formulations

Store at -20C in powder form. Store at -80C once reconstituted.
This product is supplied lyophilized. Purity is >= to 97% (HPLC)
Reconstitution Instructions
Reconstitute with DMSO or water to desired concentration.


Note: Tat-L11 and Tat-D11, are exclusively available from Novus Biologicals (Bio-Techne's exclusive patent license: US Patent 8,802,633).

Alternate Names for Tat-Beclin 1 L11 Autophagy Inducing Peptide

  • Autophagy Inducing peptide
  • Tat-Beclin 1 peptide
  • Tat-Beclin 1
  • Tat-Beclin peptide
  • Tat-Beclin
  • Tat-L11


This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Publications for Tat-Beclin 1 Autophagy Inducing Peptide (NBP2-49886)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: In vivo, in vivo.

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Showing Publications 1 - 6 of 6.
Publications using NBP2-49886 Applications Species
Vega-Rubin-de-Celis S, Zou Z, Fernandez AF et al. Increased autophagy blocks HER2-mediated breast tumorigenesis Proc Natl Acad Sci U S A 2019 Apr 17 [PMID: 29610308] (In vivo, Human) In vivo Human
Bartolomeo R, Cinque L, De Leonibus C mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017 Oct 2 [PMID: 28872463] (In vivo, Mouse) In vivo Mouse
Peraro L, Zou Z, Makwana KM et al. Diversity-Oriented Stapling Yields Intrinsically Cell-Penetrant Inducers of Autophagy J Am Chem Soc. 2017 Jun 14 [PMID: 28414223] (In vivo, Human) In vivo Human
Franco LH, Nair VR, Scharn CR et al. The Ubiquitin Ligase Smurf1 Functions in Selective Autophagy of Mycobacterium tuberculosis and Anti-tuberculous Host Defense. Cell Host Microbe 2017 Jan 11 [PMID: 28017659] (In vivo, Mouse) In vivo Mouse
Pietrocola F, Pol J Vacchelli E et al. Caloric Restriction Mimetics Enhance Anticancer Immunosurveillance Cancer Cell. 2016 Jul 11 [PMID: 27411589] (In vivo, Mouse) In vivo Mouse
Shoji-Kawata S, Sumpter R, Leveno M et al. Identification of a candidate therapeutic autophagy-inducing peptide. Nature. 2013 Feb 14 [PMID: 23364696] (in vivo)

Tat-beclin 1 (L-amino acid) / Tat-Beclin 1 L11 (NBP2-49886) and Tat–beclin 1 (D-amino acid)/Tat-Beclin 1 D11, Retroinverso form (NBP2-49888) along with the control peptide Tat-scrambled (L-amino acid)/Tat-Beclin 1 L11S Peptide, Scrambled Control (NBP2-49887) were tested in-vivo for the induction of autophagy. 6-week-old GFP-LC3 transgenic mice, and normal or CHIKV and WNV Egypt strain virus infected 5-day-old GFP-LC3 mice were injected intra-peritoneally (i.p.) with these Tat-beclin derivative peptides at a dose of 20 mg/Kg body weight (5.3uM/Kg). Brain tissues were analyzed using IHC-Frozen and Western blot analysis for measuring cell death (TUNEL Assay) and p62 expression respectively. Toxicity of these peptides was assessed in 6-day-old C57BL/6J mice via daily injections of Tat-scrambled (L-amino acid, Tat-Beclin 1 L11S) or Tat-beclin 1 (L-amino acid, Tat-Beclin 1 L11) at 15 mg kg-1 and Tat–beclin 1 (D-amino acid, Tat-Beclin 1 D11) at a dose of 20 mg/Kg body weight for 2 weeks (Figure 4).
in vivo

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