TAG-72 Antibody (B72.3 + CC49)

Immunohistochemistry-Paraffin: TAG-72 Antibody (B72.3 + CC49) [NBP2-34242] - Analysis using the Azide and BSA Free version of NBP2-34242. Staining of human colon cancer stained with TAG-72 MAb (CC49), using HRPO-DAB. Note cytoplasmic and cell surface staining.

Immunohistochemistry-Paraffin: TAG-72 Antibody (B72.3 + CC49) [NBP2-34242] - Analysis using the Azide and BSA Free version of NBP2-34242. Staining of human Ovarian Carcinoma stained with TAG-72 MAb (B72.3 + CC49).

Immunohistochemistry-Paraffin: TAG-72 Antibody (B72.3 + CC49) [NBP2-34242] - Analysis using the Azide and BSA Free version of NBP2-34242. Staining of human Prostate Carcinoma stained with TAG-72 MAb (B72.3 + CC49).

• Reactivity: Hu, Rt, Bv, Ca
• Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
• Clone: B72.3 + CC49
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TAG-72 Antibody (B72.3 + CC49) Summary

• Immunogen: Membrane-enriched fraction of a human breast carcinoma liver metastasis (B72.3); Purified TAG-72 protein (CC49)
• Localization: Cytoplasmic and cell surface
• Specificity: Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
• Application Notes: Immunohistochemistry (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues)
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS with 0.05% BSA.
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for TAG-72 Antibody (B72.3 + CC49)

• CA 72-4
• Protein TAG-72
• tag 72
• tag-72 Protein TAG-72

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.