Reactivity | Hu, Rt, Bv, CaSpecies Glossary |
Applications | WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP |
Clone | B72.3 + CC49 |
Clonality | Monoclonal |
Host | Mouse |
Conjugate | Unconjugated |
Immunogen | Membrane-enriched fraction of a human breast carcinoma liver metastasis (B72.3); Purified TAG-72 protein (CC49) |
Localization | Cytoplasmic and cell surface |
Specificity | Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. |
Isotype | IgG |
Clonality | Monoclonal |
Host | Mouse |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues) |
Theoretical MW | 220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C. |
Buffer | PBS with 0.05% BSA. |
Preservative | 0.05% Sodium Azide |
Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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