TACS-XL In Situ Apoptosis Kit [HRP]

Immunohistochemistry-Paraffin: TACS-XL In Situ Apoptosis Kit [HRP] [NB4828-30-BK] - TACS-XL In Situ Apoptosis Kit [TACS Blue Label] [NB4828-30-BK] - Mouse lung parenchyma tissue was fixed and embedded in paraffin. Five micron sections were prepared and immobilized on slides. The tissue was labeled as describe in the protocol (NB4828-30-BK) and analyzed by light microscopy for DNA fragmentation indicated by the blue stained cells. Data was provided courtesy of F. D'Agostini, University of Genova Via A. Pastore, Genova, Italy.

Product Discontinued

TACS-XL In Situ Apoptosis Kit [HRP] Summary

• Description: The TACS-XL® In Situ Apoptosis Detection Kit - Blue Label is a TUNEL assay designed for fixed cells, embedded tissue, and frozen tissue using light and electron microscopy. This kit utilizes a novel approach for the detection of apoptosis that is based on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that form during apoptosis. This detection system uses a biotin conjugated anti-BrdU antibody and streptavidin-horseradish peroxidase.
  
  Detection: BrdU/Conjugated BrdU Antibody/Streptavidin-HRP/ Blue Label
  Testing Format: Light Microscopy, Electron Microscopy
  Sample Type: Fixed cells, Embedded tissues, Frozen tissues
• Kit Type: Apoptosis Kit

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Background

Apoptosis is characterized by a number of intracellular phenomena such as membrane blebbing, chromatin condensation and nuclear DNA fragmentation. Detection of nuclear DNA fragmentation is a widely accepted method to assay for apoptosis and can be performed in situ by incorporating labeled nucleotides onto the free 3' OH ends of DNA fragments using terminal deoxynucleotidyl transferase enzyme (TdT). This type of assay, often referred to as the TUNEL assay, allows monitoring of apoptosis in cell samples or in tissue sections, providing histological localization of apoptotic events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.