TACS-XL In Situ Apoptosis Kit [HRP]

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Immunohistochemistry-Paraffin: TACS-XL In Situ Apoptosis Kit [HRP] [NB4828-30-BK] - TACS-XL In Situ Apoptosis Kit [TACS Blue Label] [NB4828-30-BK] - Mouse lung parenchyma tissue was fixed and embedded in paraffin. Five ...read more

Product Details

Summary
Applications IHC, IHC-P
Conjugate
HRP

Order Details

TACS-XL In Situ Apoptosis Kit [HRP] Summary

Description
The TACS-XL® In Situ Apoptosis Detection Kit - Blue Label is a TUNEL assay designed for fixed cells, embedded tissue, and frozen tissue using light and electron microscopy. This kit utilizes a novel approach for the detection of apoptosis that is based on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that form during apoptosis. This detection system uses a biotin conjugated anti-BrdU antibody and streptavidin-horseradish peroxidase.

Detection: BrdU/Conjugated BrdU Antibody/Streptavidin-HRP/ Blue Label
Testing Format: Light Microscopy, Electron Microscopy
Sample Type: Fixed cells, Embedded tissues, Frozen tissues
Kit Type
Apoptosis Kit

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 10X TdT Labeling Buffer
  2. 10X TdT Stop Buffer
  3. Anti-BrdU Antibody
  4. B-dNTP Mix
  5. Cytonin(TM)
  6. Nuclear Fast Red Counterstain
  7. Proteinase K Solution
  8. Strep-Diluent
  9. Strep-HRP
  10. TACS Blue Label (TM) Solution
  11. TACS-Nuclease (TM) Buffer
  12. TACS-Nuclease (TM)
  13. TdT Enzyme

Background

Apoptosis is characterized by a number of intracellular phenomena such as membrane blebbing, chromatin condensation and nuclear DNA fragmentation. Detection of nuclear DNA fragmentation is a widely accepted method to assay for apoptosis and can be performed in situ by incorporating labeled nucleotides onto the free 3' OH ends of DNA fragments using terminal deoxynucleotidyl transferase enzyme (TdT). This type of assay, often referred to as the TUNEL assay, allows monitoring of apoptosis in cell samples or in tissue sections, providing histological localization of apoptotic events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Other Available Formats

HRP NB4828-30-BK

Additional TACS-XL In Situ Products

Array NB4828-30-BK

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