T24 Overexpression Lysate Summary
T24 (Transitional-Cell Human Bladder Carcinoma)
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Supplied in SDS sample buffer containing 5% Beta-mercaptoethanol.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
Lysate Details for T24
GROWTH MEDIA: DMEM & 10% FBS (fetal bovine serum)
T24 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The T24 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% Beta-mercaptoethanol.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Publications for T24 Lysate (NBP2-03958) (0)
There are no publications for T24 Lysate (NBP2-03958).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for T24 Lysate (NBP2-03958) (0)
There are no reviews for T24 Lysate (NBP2-03958).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for T24 Lysate (NBP2-03958) (0)
Additional T24 Products
Blogs on T24