Sulfotyrosine Antibody (7C5) - BSA Free

Western Blot: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Western Blot analysis of Sulfotyrosine-BSA Conjugate showing detection of 67 kDa Sulfotyrosine protein using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 (NBP2-59384). Lane 1: Molecular Weight Ladder (MW). Lane 2: Sulfotyrosine-BSA. Lane 3: Tyrosine-BSA. Lane 4: Phosphotyrosine-BSA. Lane 5: BSA. Load: 2 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody (NBP2-59384) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Immunocytochemistry/Immunofluorescence: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5. Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

Immunohistochemistry: Sulfotyrosine Antibody (7C5) [NBP2-59384] - Immunohistochemistry analysis using Mouse Anti-Sulfotyrosine Monoclonal Antibody, Clone 7C5 (NBP2-59384). Tissue: kidney. Species: Human. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Sulfotyrosine Monoclonal Antibody (NBP2-59384) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Sulfotyrosine Antibody (D) Composite

• Reactivity: All-NA
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 7C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Sulfotyrosine Antibody (7C5) - BSA Free Summary

• Immunogen: Synthetic Sulfotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry 1:25
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Sulfotyrosine Antibody (7C5) - BSA Free

• sTYR;S-Tyrosine;Sulfated Tyrosine;Sulfotyrosine;Sulphated Tyrosine;Sulphotyrosine;Tyrosine sulfation;Tyrosine Sulphation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sulfotyrosine Antibody (NBP2-59384)(1)

Publication using NBP2-59384

• Xu P, Xi Y, Zhu J et al. Intestinal Sulfation Is Essential to Protect Against Colitis and Colonic Carcinogenesis Gastroenterology 2021-04-02 [PMID: 33819483] (IF/IHC)