STK32A Antibody (3E8-1D3) - Azide and BSA Free

Western Blot: STK32A Antibody (3E8-1D3) [H00202374-M01] - Analysis of STK32A over-expressed 293 cell line, cotransfected with STK32A Validated Chimera RNAi ( Cat # H00202374-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK32A monoclonal antibody (M01) clone 3E8-1D3 (Cat # H00202374-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: STK32A Antibody (3E8-1D3) [H00202374-M01] - Analysis of STK32A expression in transfected 293T cell line by STK32A monoclonal antibody (M01), clone 3E8-1D3.Lane 1: STK32A transfected lysate(19.8 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, KD
• Clone: 3E8-1D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

STK32A Antibody (3E8-1D3) - Azide and BSA Free Summary

• Immunogen: STK32A (AAH21666, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGANTSRKPPVFDENEDVNFDHFEILRAIGKGSFGKVCIVQKNDTKKMYAMKYMNKQKCVERNEVRNVFKELQIMQGLEHPFLVNLWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVHFKEETVKLFICELVMALDYLQNQRIIHRDMKPDNILLDEHDTWLSYKSH
• Localization: Cytoplasm. Cell membrane. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).
• Specificity: STK32A - serine/threonine kinase 32A
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: STK32A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for western blot. It has also been used for RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for STK32A Antibody (3E8-1D3) - Azide and BSA Free

• A930015B13Rik
• EC 2.7.11
• EC 2.7.11.1
• MGC22688
• serine/threonine kinase 32A
• YANK1serine/threonine-protein kinase 32A

Background

Sprouty was originally identified as an inhibitor of Drosophila epidermal growth factor (EGF) and fibroblast growth factor (FGF) receptor signaling during tracheal development. Four isoforms of mammalian sprouty are known (Spry1-Spry4). The role of the mammalian analogs has not been clearly elucidated although it is believed that human Sprouty 2 (hSpry2) may be an inhibitor of cellular migration and proliferation. Spry2 and Spry4 show considerable sequence homology between human and mouse at the C terminus of the protein. Significant sequence divergence occurs at the N terminus. Spry2 appears to be abundant in brain, lung and heart tissue, with lesser amounts found in kidney and skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STK32A
• Entrez:
  • 202374(Human)
• Uniprot:
  • AAH21666(Human)