SSEA-4 Antibody (MC-813-70) - Azide and BSA Free

Flow Cytometry: SSEA-4 Antibody (MC-813-70) - Azide and BSA Free [NBP2-80963] - Cell surface analysis on 2102Ep cells using 0.1 ug/10^6 cells of NBP2-26644 (green histogram represents isotype control; red represents SSEA-4 antibody). Image from the standard format of this antibody.

Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) - Azide and BSA Free [NBP2-80963] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-26644. Staining of SSEA-4 on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26647 (green histogram represents isotype control; red represents SSEA-4 antibody).

Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) - Azide and BSA Free [NBP2-80963] - Analysis using the FITC conjugate of NBP2-26644. Staining of SSEA-4 on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26645 (green histogram represents isotype control; red represents SSEA-4 antibody).

Flow Cytometry: SSEA-4 Antibody (MC-813-70) - Azide and BSA Free [NBP2-80963] - Cell surface flow cytometric analysis of SSEA-4 PE on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26648 (green histogram represents isotype control; red represents SSEA-4 antibody). Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Ca, Ch, Pm, Rb
• Applications: Flow, ICC/IF, IHC
• Clone: MC-813-70
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.4 mg/ml

SSEA-4 Antibody (MC-813-70) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse SSEA-4 Antibody (MC-813-70) - Azide and BSA Free (NBP2-26644) is a monoclonal antibody validated for use in IHC, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human embryonal carcinoma cell line 2102Ep
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 8-25 ug/ml. Use reported in scientific literature (PMID 28859517)
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.4 mg/ml
• Purity: Protein G purified

Alternate Names for SSEA-4 Antibody (MC-813-70) - Azide and BSA Free

• SSEA4
• SSEA-4
• SSEA-4;stage-specific embryonic antigen 4

Background

SSEA-4 specific antibody, clone MC-813, is reactive with Stage-Specific Embyronic Antigen-4, a carbohydrate antigen expressed on human teratocarcinoma cells (EC), embryonic stem cells (ES) and embryonic germ cells. It is also expressed on a subset of mesenchymal stem cells, erythrocytes, the erythroleukemic myeloid cell line K562, unfertilized oocytes and early cleaved stage oocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.