SSEA-4 Antibody (MC-813-70) - BSA Free

Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Analysis using the FITC conjugate of NBP2-26644. Staining of SSEA-4 on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26645 (green histogram represents isotype control; red represents SSEA-4 antibody).

Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Cell surface analysis on 2102Ep cells using 0.1 ug/10^6 cells of NBP2-26644 (green histogram represents isotype control; red represents SSEA-4 antibody).

Flow Cytometry: SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Cell surface flow cytometric analysis of SSEA-4 PE on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26648 (green histogram represents isotype control; red represents SSEA-4 antibody).

Flow (Cell Surface): SSEA-4 Antibody (MC-813-70) [NBP2-26644] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-26644. Staining of SSEA-4 on human 2102Ep cells using 10 ul/10^6 cells of NBP2-26647 (green histogram represents isotype control; red represents SSEA-4 antibody).

• Reactivity: Hu, Mu, Ca, Ch, Pm, Rb
• Applications: WB, Flow, ICC/IF, IHC
• Clone: MC-813-70
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SSEA-4 Antibody (MC-813-70) - BSA Free Summary

• Immunogen: Human embryonal carcinoma cell line 2102Ep
• Marker: Embryonic Stem Cell Marker
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 28859517)
  • Immunohistochemistry
  • Western Blot reported in scientific literature (PMID 28302994)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for SSEA-4 Antibody (MC-813-70) - BSA Free

• SSEA4
• SSEA-4
• SSEA-4;stage-specific embryonic antigen 4

Background

SSEA-4 specific antibody, clone MC-813, is reactive with Stage-Specific Embyronic Antigen-4, a carbohydrate antigen expressed on human teratocarcinoma cells (EC), embryonic stem cells (ES) and embryonic germ cells. It is also expressed on a subset of mesenchymal stem cells, erythrocytes, the erythroleukemic myeloid cell line K562, unfertilized oocytes and early cleaved stage oocytes. This antibody was first categorized in the following publication: PMID 6141938 https://pubmed.ncbi.nlm.nih.gov/6141938/

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSEA-4 Antibody (NBP2-26644)(2)

Publications using NBP2-26644

• Huang H, Xu H, Zhao J. A Novel Approach for Meniscal Regeneration Using Kartogenin-Treated Autologous Tendon Graft Am J Sports Med 2017-08-01 [PMID: 28859517] (ICC/IF, Rabbit)
• Yu Yang, Lin Lixiang, Zhou Yifei et al. Effect of Hypoxia on Self-Renewal Capacity and Differentiation in Human Tendon-Derived Stem Cells. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research 2017-03-17 [PMID: 28302994] (WB, Human)