SSEA-3 Antibody (MC-631) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
MC-631
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

SSEA-3 Antibody (MC-631) [Alexa Fluor® 594] Summary

Immunogen
Four to eight cell stage mouse embryos
Specificity
Recognizes a carbohydrate epitope of SSEA-3 (4, 5).
Isotype
IgM
Clonality
Monoclonal
Host
Rat
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SSEA-3 Antibody (MC-631) [Alexa Fluor® 594]

  • SSEA3
  • SSEA-3
  • Stage specific embryonic antigen 3
  • Stage-specific embryonic antigen 3

Background

SSEA-3, also known as glycolipid GB5, is expressed on the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES) (1). Expression of SSEA-3 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-3 expression (2, 3).

  1. Zhou, D. et al. (2000) J. Biol. Chem. 275:22631.
  2. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  3. Draper, J.S. et al. (2002) J. Anat. 200:249.
  4. Shevinsky, L.H. et al. (1982) Cell 30:697.
  5. Kannagi, R. et al. (1983) EMBO J. 2:2355.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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