![ELISA: ssDNA Antibody (TNT-3) [NBP2-29849] - Various dilutions of single stranded DNA from calf thymus (starting at 10 mg/mL) were analyzed with 0.5 mg of, Anti-DNA, single stranded, clone TNT-3. Alkaline phosphatase-conjugated Goat Anti-Mouse secondary antibody was used for detection.](http://images.novusbio.com/fullsize/ssDNA-Antibody-TNT-3-ELISA-NBP2-29849-img0002.jpg "ELISA: ssDNA Antibody (TNT-3) [NBP2-29849] - Various dilutions of single stranded DNA from calf thymus (starting at 10 mg/mL) were analyzed with 0.5 mg of, Anti-DNA, single stranded, clone TNT-3. Alkaline phosphatase-conjugated Goat Anti-Mouse secondary antibody was used for detection.")
| Reactivity | AllSpecies Glossary |
| Applications | Simple Western, ELISA, Flow, ICC/IF, IHC |
| Clone | TNT-3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Immunogen | Human Raji cell nuclei. |
| Specificity | Reacts with single stranded DNA (ssDNA). The antibody is useful for staining nucleated cells. The antibody can also be used to identify necrotic regions of tumors and damaged tissues. By immunohistochemistry, stains heterochromatic regions of cell nucleus. |
| Isotype | IgG3 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ICC/IF on paraformaldehyde fixed cells Immunohistochemistry on paraformaldehyde or B5 fixed tissue sections. Flow cytometry: using 2% paraformaldehyde fixed cells. Elisa staining pattern: Stains heterochromatic regions of cell nucleus. Optimal working dilutions must be determined by end user. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
| Publication using NBP2-29849 | Applications | Species |
|---|---|---|
| Yasuhara T, Kato R, Yamauchi M et al. RAP80 suppresses the vulnerability of R-loops during DNA double-strand break repair Cell reports 2022-02-01 [PMID: 35108530] |
Secondary Antibodies |
Isotype Controls |
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