Recombinant SARS-CoV-2 Spike S2 HR1 His6-SUMO (N-term) Protein

• Reactivity: V

Recombinant SARS-CoV-2 Spike S2 HR1 His6-SUMO (N-term) Protein Summary

• Description: A partial recombinant protein with an N-terminal His6-SUMO tag corresponding to SARS-CoV-2 Spike S2 Heptad Repeat 1.

  Source: E.coli

  Amino Acid Sequence: Gly910-Glu988

• Source: E. coli
• Protein/Peptide Type: Partial Recombinant Protein
• Gene: S
• Purity: >95% pure by SDS-PAGE

Applications/Dilutions

• Theoretical MW: 20.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: 0.2 uM filtered solution of PBS, pH 7.4
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant SARS-CoV-2 Spike S2 HR1 His6-SUMO (N-term) Protein

• COVID-19 Spike S2 HR1
• S2 Protein Heptad Repeat 1
• SARS-CoV-2 Spike S2 Heptad Repeat 1
• Spike glycoprotein Subunit 2 HR1
• Spike S2 4,3 hydrophobic repeat region 1

Background

The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. After binding of RBD in S1 subunit of S protein on the virion to the ACE2 receptor on the target cell, the heptad repeat 1 (HR1) and 2 (HR2) domains in its S2 subunit of S protein interact with each other to form a six-helix bundle (6-HB) fusion core, bringing viral and cellular membranes into close proximity for fusion and infection. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: S