Smith Antigen Antibody (Y12)

Images

 
Western Blot: Smith Antigen Antibody (Y12) [NB600-546] - Analysis of LS174T Cells.
Immunohistochemistry-Paraffin: Smith Antigen Antibody (Y12) [NB600-546] - Human tonsil stained with Smith antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Dr, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IM
Clone
Y12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Smith Antigen Antibody (Y12) Summary

Immunogen
The immunogen is generated from human Smith Antigen.
Localization
Nuclear
Specificity
Ab-1 recognizes cross-reactive epitopes on the B'/B and D polypeptides of Sm. Ab-1 precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all these RNA-protein complexes.
Isotype
IgG3 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:50 - 1:100
  • Immunomicroscopy 1:10 - 1:500
  • Western Blot 0.5 - 1.0 ug/ml
Application Notes
WB: Detects an approx. 25 kDa, which corresponds to the predicted molecular weight of Smith Antigen.Precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all of these RNA-protein complexes. Use in ELISA reported in scientific literature (PMID 28093526). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28515487).
Publications
Read Publications using
NB600-546 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 17473171). Canine (PMID: 10330151). Mouse reactivity reported in scientific literature (PMID: 28093526).Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for Smith Antigen Antibody (Y12)

  • Smith antigen

Background

The sm (Smith) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE). Antibodies to sm are found in 20-30% of patients with SLE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smith Antigen Antibody (NB600-546)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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