Smallpox B5R Antibody (1J2F3) [PerCP]

• Reactivity: V-Vi
• Applications: WB
• Clone: 1J2F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP

Smallpox B5R Antibody (1J2F3) [PerCP] Summary

• Immunogen: Amino acids 33-47 (IQEYNLIRTYKKVDK) ofthe B5R protein were used as the immunogen (NCBI Reference Sequence: NP_042218.1)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: B7R
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: The amino acid sequence used as immunogen for this antibody has the following sequence identity with various virsues: 100% with variola virus, 93% with cowpox, camelpox and taderapox virus, 86% with vaccinia virus, and 80% with ectromelia, rabbitpox and monkeypox.

Reactivity Notes

The amino acid sequence used as immunogen for this phospho antibody is 100% homologous in human, chimpanzee, dog, rat, mouse, chicken and zebrafish and 92% homologous in xenopus.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Smallpox B5R Antibody (1J2F3) [PerCP]

• B5R protein
• B6R
• B7R protein
• B7R
• EEV membrane glycoprotein
• Glycoprotein
• H7R protein
• H7R
• ORF5R
• VARVgp175

Background

Vaccinia virus, a member of Poxviridae family, is a dsDNA enveloped virus. It consists of 4 types of virion stages among which extracellular enveloped virus (EEV) is critical for cell-to-cell and long range viral infection. B5R is an EEV-specific Type I membrane glycoprotein essential for EEV formation. It consists of a large ectodomain containing regions similar to SCR domains of complement regulatory proteins followed by a transmembrane region and a cytoplasmic tail. B5R is essential for efficient wrapping of IMV, actin tail formation, normal plaque size, virus virulence, incorporation of the protein into EEV and for EEV formation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B7R