SARS Nucleocapsid Protein Antibody (001) [Alexa Fluor® 488]

Product Image: SARS Nucleocapsid Protein Antibody (001) [Alexa Fluor® 488] [NBP2-90988AF488] - Vial of Alexa Fluor 488 conjugated antibody. Alexa Fluor 488 is optimally excited at 490 nm by the Blue laser (488 nm) and has an emission maximum of 525 nm.

• Reactivity: V, V
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 001
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 488

SARS Nucleocapsid Protein Antibody (001) [Alexa Fluor® 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant SARS-CoV Nucleoprotein/NP Protein
• Specificity: Has cross-reactivity in ELISA and WB with:
  SARS-CoV-2 Nucleocapsid Protein
  
  Has cross-reactivity in ELISA with:
  SARS-CoV-2 Omicron (B.1.1.529) Nucleocapsid Protein
  SARS-CoV-2 Nucleocapsid Protein
  SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(R203K, G204R) Protein
  SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(I292T) Protein
  SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, R203K, G204R, S235F) Protein
  SARS-CoV-2 Alpha(B.1.1.7) Nucleocapsid(D3L, S235F) Protein
  SARS-CoV-2 Alpha(B.1.1.7/ B.1.237) Nucleocapsid(S194L) Protein
  SARS-CoV-2 Alpha/beta(B.1.1.7/ B.1.351/ A.2.2) Nucleocapsid(P13L) Protein
  SARS-CoV-2 Beta(B.1.351/B.1.351.2/b.1.351.3/B.1.427/B.1.429) Nucleocapsid (T205I) Protein
  SARS-CoV-2 Gamma(P.1/ P.1.1/ P.1.2) Nucleocapsid (P80R) Protein
  SARS-CoV-2 Eta(B.1.525) Nucleocapsid (A12G, T205I) Protein
  SARS-CoV-2 (B.1.617) Nucleocapsid (D377Y) Protein
  SARS-CoV-2 (B.1.617) Nucleocapsid (R203M, D377Y) Protein
  SARS-CoV-2 Delta(B.1.617.2) Nucleocapsid (D63G, R203M, D377Y) Protein
  SARS-CoV-2 (B.1.617.3) Nucleocapsid (P67S, R203M, D377Y) Protein
  
  No cross-reactivity in ELISA with:
  MERS-CoV Nucleocapsid protein
  HCoV-229E Nucleocapsid protein
  HCoV-NL63 Nucleocapsid protein
  HCoV-HKU1(isolate N5) Nucleocapsid protein
  HCoV-OC43 Nucleocapsid protein
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: N
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-90988. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

This antibody has cross-reactivity in ELISA and WB with:
SARS-CoV-2 Nucleocapsid Protein

No cross-reactivity in ELISA with:
MERS-CoV Nucleoprotein protein
HCoV-229E Nucleoprotein protein
HCoV-NL63 Nucleoprotein protein
HCoV-HKU1(isolate N5) Nucleoprotein protein
HCoV-OC43 Nucleoprotein

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SARS Nucleocapsid Protein Antibody (001) [Alexa Fluor® 488]

• N protein
• N structural protein
• N
• NC
• Nucleocapsid protein
• Nucleoprotein
• Protein N
• SARS coronavirus N protein
• SARS coronavirus nucleocapsid protein
• SARS CoV N protein
• SARS CoV nucleocapsid protein
• SARS CoV
• SARS N protein
• SARS Nucleoprotein
• SARSCoV N protein
• SARSCoV nucleocapsid protein
• SARSCoV
• Severe acute respiratory syndrome

Background

It has recently been shown that SARS is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: N