Sambucus Nigra Lectin [Cy5]

Immunofluorescence: Sambucus Nigra Lectin [Cy5] [CL-1305-NB] - Colon FFPE stained with Cy5 Sambucus Nigra Lectin (fuchsia) and DAPI (blue).

• Applications: ICC/IF
• Conjugate: Cy5
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Sambucus Nigra Lectin [Cy5] Summary

• Description: Features:
  
  
• Cy5 labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.
• This conjugate is supplied essentially free of unconjugated fluorochromes.
• Excitation: 650 nm
• Emission: 670 nm
• Color: Far Red

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4C, warm to 37C and centrifuge before use. The recommended concentration range for use is 5-20 ug/mL..

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM Ca^2+, 0.01 mM Mn^2+, protein stabilizer.
• Preservative: 0.08% Sodium Azide
• Concentration: Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in alpha-2,6 and to a lesser degree, alpha-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. Sambucus nigra lectin has been reported to inhibit cell-free protein synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for Sambucus Nigra Lectin (CL-1305-NB)(1)

Publication using CL-1305-NB

• Rao TC, Beggs RR, Ankenbauer KE et al. ST6Gal-I-mediated sialylation of the epidermal growth factor receptor modulates cell mechanics and enhances invasion The Journal of biological chemistry 2022-02-11 [PMID: 35157848]