Sambucus Nigra Lectin [Cy5]

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Immunofluorescence: Sambucus Nigra Lectin [Cy5] [CL-1305-NB] - Colon FFPE stained with Cy5 Sambucus Nigra Lectin (fuchsia) and DAPI (blue).

Product Details

Summary
Applications ICC/IF
Conjugate
Cy5
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Order Details

Sambucus Nigra Lectin [Cy5] Summary

Description
Features:

  • Cy5 labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.
  • This conjugate is supplied essentially free of unconjugated fluorochromes.
  • Excitation: 650 nm
  • Emission: 670 nm
  • Color: Far Red

  • Applications/Dilutions

    Application Notes
    A precipitate may form during storage. This does not have a significant adverse effect on the product. If a precipitate forms upon long-term storage at 4C, warm to 37C and centrifuge before use. The recommended concentration range for use is 5-20 ug/mL.

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark.
    Buffer
    10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.1 mM Ca^2+, 0.01 mM Mn^2+, protein stabilizer.
    Preservative
    0.08% Sodium Azide
    Concentration
    Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in alpha-2,6 and to a lesser degree, alpha-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin does not appear to bind sialic acid linked to N-acetylgalactosamine. Sambucus nigra lectin has been reported to inhibit cell-free protein synthesis.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Other Available Formats

    Cy5 CL-1305-NB

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