![Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-S-Tag antibody NB600-529 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-529-img0004.jpg "Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-S-Tag antibody NB600-529 used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Goat S-Tag Epitope Tag Antibody - BSA Free (NB600-529) is a polyclonal antibody validated for use in WB, ELISA, ICC/IF and IP. Anti-S-Tag Epitope Tag Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Goats were immunized with S-Tag (KETAAAKFERQHMDS) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500 |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Analysis using the HRP conjugate of NB600-529. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).](http://images.novusbio.com/fullsize/S-Tag-Epitope-Tag-Antibody-Western-Blot-NB600-529-img0003.jpg "Western Blot: S-Tag Epitope Tag Antibody [NB600-529] - Analysis using the HRP conjugate of NB600-529. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).")
