Rubisco Antibody

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Western Blot: Rubisco Antibody [NB110-81553] - Purified spinach Rubisco protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development ( ...read more

Product Details

Summary
Reactivity PlSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Rubisco Antibody Summary

Immunogen
1-491
Specificity
Rubisco
Clonality
Polyclonal
Host
Chicken
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
Western Blot: Purified spinach Rubisco protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development ( anti-rbcL )

Reactivity Notes

Spinach.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rubisco Antibody

  • 5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I [Spinacia oleracea]
  • EC 2.1.1.43
  • rbcL
  • Ribulose-1

Background

Rubisco is an enzyme that is used in the Calvin cycle to catalyze the first major step of carbon fixation, a process by which the atoms of atmospheric carbon dioxide are made available to organisms in the form of energy-rich molecules such as sucrose. Rubisco catalyzes either the carboxylation or oxygenation of ribulose-1,5-bisphosphate (also known as RuBP) with carbon dioxide or oxygen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Rubisco Antibody (NB110-81553). (Showing 1 - 1 of 1 FAQ).

  1. Our customer would like to ensure this antibody can recognize Rubisco from Nicotiana Benthamiana. Would you please help confirm?
    • The immunogen for this antibody shares just 63% similarity with rubisco from Nicotiana species. It is not predicted to cross-react.
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