Rotavirus Inner Capsid Protein Antibody (A2)

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Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ICC/IF
Clone
A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Rotavirus Inner Capsid Protein Antibody (A2) Summary

Immunogen
Recombinant inner capsid protein (VP6).
Specificity
The mouse monoclonal reacts specifically with human 41 kDa inner capsid protein (VP6) of Rotavirus.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:200 to 1:1 000
  • Immunocytochemistry/Immunofluorescence 1:100 to 1:500
Publications
Read Publication using
NB110-37243 in the following applications:

  • WB
    1 publication

Reactivity Notes

Virus reactivity reported in scientific literature (PMID:33067651).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.1M Tris, 0.1M glycine, and 2.0% sucrose.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Lyophilized powder, reconstituted in distilled water.

Background

Rotaviruses, members of the family Reoviridae, are a major cause of diarrhea in young mammals. Rotavirus infections also result in economic losses in agriculture due to diarrhea in calf, pig, sheep, and poultry rearing. Diarrhea (or scours) due to the rotavirus Nebraska Calf Diarrhea Virus can affect calves up to 30 days of age or older. Diarrhea begins 2 to 3 days after exposure. Diagnosis is by history, lesions (ulcers on the tongue, lips, and mouth) and laboratory tests. Mortality rates may be as high as 50 percent, depending on the secondary bacteria present. Human rotaviruses, the major etiological agents of severe infantile diarrhea worldwide, display very diverse and complex serotypic specificities. Rotaviruses are 70 nm, non enveloped viruses comprised of a triple layered protein capsid; Outer capsid proteins are VP4 and VP7, Inner capsid -VP6 and Core -VP2. The immunity acquired from exposure to rotavirus appears to be type specific following initial infection. Therefore, multiple serotypes of rotavirus mean multiple opportunities for infection. The combination of animal reservoirs for the virus and rotavirus gene reassortment provides the potential for dramatic genetic shifts (similar to influenza virus) which could give rise to altered host ranges and viral virulence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rotavirus Inner Capsid Protein Antibody (NB110-37243)(1)

We have publications tested in 1 confirmed species: Virus.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rotavirus Inner Capsid Protein Antibody (NB110-37243) (0)

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Secondary Antibodies

 

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