Ricinus Communis Agglutinin 60 Antibody (CP75)

Product Discontinued

Ricinus Communis Agglutinin 60 Antibody (CP75) Summary

• Immunogen: Natural RCA60, full length.
• Localization: Nuclear
• Specificity: Recognizes the A chain of RCA60. Also recognizes RCA120.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: WB: Predicted molecular weight: 63 kDa. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross reacts with Ricinus communis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Ricinus Communis Agglutinin 60 Antibody (CP75)

• Agglutinin
• Castor bean
• RCA
• RCA60
• Ricn

Background

Ricinus communis agglutinin 60(RCA60) is an extremely toxic glycoprotein consisting of two disulfide-linked chains of about 28,000 daltons and 32,000 daltons, termed A and B chain, respectively. The B chain can bind to cell surfaces via galactose or N-acetylgalactosamine residues of membrane glycoconjugates and facilitates the transport of the lectin into the cell. The A chain has an enzyme activity which can catalytically block protein synthesis and is so toxic that only a single molecule of A chain is required to kill a cell. Alone, the A chain is incapable of entering the cell and is thus not toxic. The A chain can be separated from the B chain and then linked by a cleavable disulfide bond to other proteins, such as antibodies. These antibody-toxin hybrid molecules can be used to kill specific cells with the appropriate antigenic determinant exposed, such as tumor markers on surfaces of malignant cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.