Reticulum Cells Antibody (ED11) - BSA Free

Product Discontinued

Reticulum Cells Antibody (ED11) - BSA Free Summary

• Immunogen: Rat spleen cell homogenate
• Specificity: NB100-64723 recognizes reticular elements in the T cell areas and B cell follicles of spleen, lymph nodes, Peyers patches, bronchus and gut associated lymphoid tissues (BALT and GALT). In the thymus a reticular pattern is observed throughout the cortex capsule, the medulla is negative. In non-lymphoid organs positivity is seen only in reticular elements of the small intestine. No staining of liver, lung, skin, kidney, brain, pancreas or heart is seen. Biochemical analysis has revealed that ED11 recognizes complement factor C3. in vivo studies have shown that ED11 blocks immune complex trapping on follicular dendritic cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:300
• Application Notes: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Background

Dendritic cells (DCs) are known as the professional antigen presenting cells. DCs perform the important role of capturing and processing antigen and presenting it to native T-helper cells to initiate the immune response. Distinct subsets of DCs have been described, they include interdigitating reticulum cells. The antigen recognized is found on a subpopulation (approximately 5%) of circulating monocytes and peritoneal macrophages, as well as on in vitro LPS and IFN stimulated macrophages and on all macrophages after 3 weeks in culture. It is absent from all other blood cells. Detects a subpopulation of reticulum cells in T-cell areas of lymph nodes and spleen and their in vitro correlates. It is also useful for the detection of a macrophage subpopulation in the thymus. A minor but distinct population of leukocytes in the kidney cortex is also positively stained by this antibody. The antigen is absent in the skin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.