Reg1A Antibody

Images

 
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Reg1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4937) followed by HRP-conjugated ...read more
Reg1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Sheep Anti-Human Reg1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4937) at 3 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Reg1A Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Reg1A
Gln23-Asn166
Accession # P05451
Specificity
Detects human Reg1A in direct ELISAs. In direct ELISAs, approximately 80% cross-reactivity with recombinant human (rh) Reg1B is observed, approximately 20% cross-reactivity with recombinant mouse Reg1, and less than 5% cross-reactivity with rhReg3A and recombinant rat Reg1A is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
REG1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg1A Antibody

  • ICRF
  • Islet cells regeneration factor
  • Islet of Langerhans regenerating protein
  • lithostathine-1-alpha
  • P19
  • pancreatic stone protein, secretory
  • Pancreatic thread protein
  • protein-X
  • PSP
  • PSPS
  • PSPS1
  • PTP
  • REG
  • Reg1A
  • REG-1-alpha
  • Regenerating islet-derived protein 1-alpha
  • Regenerating protein I alpha

Background

Reg1A (Regenerating gene IA/ alpha ; also REG, ICRF, PSPS, PTP and Lithostathine-1 alpha ) is a secreted, variably glycosylated 15-22 kDa type I member of the REG gene family of proteins. It is induced in proliferating cell types such as colonic epithelium, islet beta -cells, and multiple tumor types, and is constitutively expressed by pancreatic acinar cells. Thus, Reg1A is found both in blood (ng/mL quantities) and pancreatic secretions. It would appear that Reg1A plays a role in blocking apoptosis, and is upregulated by both IL-22 and IL-6. Human Reg1A is synthesized as a 166 amino acid (aa) precursor. It contains a 22 aa signal sequence plus a 144 aa mature region that is characterized by the presence of three intrachain disulfide bonds, one C-type lectin domain (aa 34-164), and a utilized N-terminal O-linked glycosylation site (Thr27). Variablility in the glycosylation pattern, plus proteolytic cleavage after Arg11 generates Reg1A isoforms in the 15-22 kDa range. There is one potential isoform variant that shows an alternative start site at Met9. Mature (aa 23-166) human Reg1A shares 76% and 69% aa sequence identity with mouse and rat Reg1A, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB200-106
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
AF1366
Species: Hu
Applications: WB, Simple Western, IHC
NB100-82245
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
MAB6798
Species: Hu
Applications: WB, ICC
NBP1-82454
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
H00027065-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP2-46085
Species: Hu
Applications: WB, IHC
NBP2-67321
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-33736
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NBP2-67627
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-49833
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, PEP-ELISA
NB120-3216
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, IF
AF4937
Species: Hu
Applications: WB, IHC

Publications for Reg1A Antibody (AF4937) (0)

There are no publications for Reg1A Antibody (AF4937).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Reg1A Antibody (AF4937) (0)

There are no reviews for Reg1A Antibody (AF4937). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Reg1A Antibody (AF4937) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Reg1A Products

Bioinformatics Tool for Reg1A Antibody (AF4937)

Discover related pathways, diseases and genes to Reg1A Antibody (AF4937). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Reg1A Antibody (AF4937)

Discover more about diseases related to Reg1A Antibody (AF4937).
 

Pathways for Reg1A Antibody (AF4937)

View related products by pathway.

PTMs for Reg1A Antibody (AF4937)

Learn more about PTMs related to Reg1A Antibody (AF4937).

Blogs on Reg1A

There are no specific blogs for Reg1A, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Reg1A Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol REG1A
Uniprot