Recombinant Viral 136R/Y136 Protein

• Reactivity: V
• Applications: Bioactivity

Recombinant Viral 136R/Y136 Protein Summary

• Additional Information: Type I and III Interferon Inhibitor
• Details of Functionality: Measured by its ability to inhibit Type-I and Type-III IFN-mediated anti-viral activity. Huang, J. et al. (2007) PANS 104:9822. The ED₅₀ for this effect is 2-12 ng/mL in the presence of 20 pg/mL Recombinant Human IFN-beta (Catalog # 8499-IF).
• Source: Chinese Hamster Ovary cell line, CHO-derived viral 136R protein
  

  Viral 136R/Y136 (Tyr17-Lys351) Accession # Q9DHH7	HPGGGSGGGSGGGS	HHHHHH
  N-terminus		C-terminus

• N-terminal Sequence: Tyr17
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 88-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Viral 136R/Y136 Protein

• 136R

Background

136R, also known as Y136, is an approximately 80 kDa glycosylated and secreted protein encoded by the Yaba-like disease virus genome (1). 136R binds directly to type I and type III interferons (IFN-alpha and IFN-lambda) (1). It prevents the interaction of rhesus and human IFN-alpha as well as human IFN-beta with the IFN-gamma R1 complex, although it shares only 14% amino acid sequence identity with the ECD of human IFN-gamma R1 (1). 136R blocks STAT1 signaling and the anti-viral protective functions of IFN-alpha, -beta, -omega, -kappa, and -lambda 1/IL-29 (1).

1. Huang, J. et al. (2007) Proc. Natl. Acad. Sci. USA 104:9822.

Publications for 136R (8976-BR)(1)

Publication using 8976-BR

• J Ardanuy, K Scanlon, C Skerry, SY Fuchs, NH Carbonetti Age-Dependent Effects of Type I and Type III IFNs in the Pathogenesis of Bordetella pertussis Infection and Disease J. Immunol., 2020-03-09;0(0):. 2020-03-09 [PMID: 32152071] (In Vivo, Mouse)