Recombinant Mouse IFN-alpha 12 Protein, CF

Recombinant Mouse IFN-alpha 12 (Catalog # 10370-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 10-100 pg/mL.

2 μg/lane of Recombinant Mouse IFN-alpha 12 (Catalog # 10370-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-25 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IFN-alpha 12 Protein, CF Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 10-100 pg/mL.
• Source: Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 12 protein
  Cys24-Glu189
• Accession #: Q80SS5.1
• N-terminal Sequence: Cys24
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW:

  19.2 kDa

  .
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18-25 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-alpha 12 Protein, CF

• IFNA12
• IFN-alpha 12
• Interferon alpha 12

Background

Interferon-alpha 12 (IFN alpha -12) is a member of the type I interferon-alpha (IFN-alpha ) family (1). The members of the IFN-alpha family, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1-3). IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (2). The mature extracellular domain (ECD) of mouse IFN alpha -12 is 166 aa. While IFNa-12 does not exist in humans, it closely resembles other murine members of the IFN-alpha family, such as sharing 88% and 86% sequence identity with IFN alpha -15 and IFN alpha -11 respectively. Murine IFN-alpha 12 differs from other members of the IFN-alpha family by its anti-proliferative function and in the inability of its expression to be regulated by viral infection (5).

1. Moulton, V. R. (2017) Trends Mol Med. 23:615.
2. Oritani, K. et al. (2001) Cytokine & Growth Factor Reviews. 12:337.
3. Pesch, V. et al. (2004). Journal of Virology. 78:8219.
4. Hardy, M. P. et al. (2004). Genomics, 84:331.
5. Tsang, S. L. et al. (2007). Cytokine, 37:101.

Bioinformatics

• Uniprot:
  • Q80SS5.1()