Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF

2 μg/lane of Recombinant Mouse CTRP4/C1qTNF4 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 34-38 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse CTRP4/C1qTNF4, immobilized at 2.5-5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
• Source: Mouse myeloma cell line, NS0-derived mouse CTRP4/C1qTNF4 protein
  Leu17-Leu326, with a C-terminal 6-His tag
• Accession #: Q8R066
• N-terminal Sequence: Leu17
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CTRP4/C1qTNF4 His-tag Protein, CF

• C1q and tumor necrosis factor related protein 4
• C1qTNF4
• complement C1q tumor necrosis factor-related protein 4
• complement-c1q tumor necrosis factor-related protein 4
• CTRP4
• CTRP4ZACRP4
• ZACRP4

Background

C1qTNF4, also known as CTRP4, is an approximately 34 kDa member of the C1q family of secreted proteins (1, 2). C1qTNF4 consists of a signal peptide followed by two tandem globular C1q domains (1). It is the only C1q family member with tandem C1q domains (1, 2). The second C1q domain contains an RGD peptide, suggesting that it could potentially interact with integrins (3). Mature mouse C1qTNF4 shares 95% and 98% amino acid sequence identity with human and rat C1qTNF4, respectively. Expression of human C1qTNF4 occurs most abundantly in brain and adipose tissue (1). Neurons in the hypothalamus express and secrete CTRP4 which modulates food intake and energy balance by controlling neuropeptide gene expression (1). In-house finding shows CTRP4 can support neurite outgrowth of cortical neurons. Like other CTRP family members, C1qTNF4 can form dimers, trimers, hexamers, and high molecular weight oligomers following secretion (1, 4). Injection of recombinant C1qTNF4 into mice can reduce food intake, body weight, and ambulatory activity levels (1). C1qTNF4 has also been shown to promote human cancer cell survival in vitro (5).

1. Byerly, M.S. et al. (2014) J. Biol. Chem. 289:4055.
2. Kishore, U. et al. (2004) Trends Immunol. 25:551.
3. Ruoslahti, E. (1996) Annu. Rev. Cell Dev. Biol. 12:697.
4. Wong, G.W. et al. (2008) Biochem. J. 416:161.
5. Li, Q. et al. (2011) Cancer Lett. 308:203.

Bioinformatics

• Uniprot:
  • Q8R066()